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PXD000480

PXD000480 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRat prefrontal cortex LC-MS/MS
DescriptionThe prefrontal cortex is greatly associated with a wide range of mental health illnesses including schizophrenia, depression, bipolar disorder, anxiety and autism spectrum disorders. It richly expresses neuroreceptors which are the target for typical and atypical antipsychotics. However as the precise mechanism of action of antipsychotic medications are not known, proteomic studies of the effects of antipsychotic drugs on the brain are warranted. In the current study we aimed to characterise protein expression in the adult rodent prefrontal cortex (n=5 per group) following low dose treatment with the atypical antipsychotic Risperidone or saline (control) in adolescence (postnatal days 34-47). The prefrontal cortex was examined by triplicate one hour runs of label-free LC-MS/MS. The raw mass spectral data were analyzed with the MaxQuantTM software. Statistical analysis was carried out using SAS Version 9.1. Functional and pathway analysis was performed with DAVID.nih and Ingenuity Pathway Analysis respectively and the top five most implicated pathways were found to be clathrin mediated endocytosis, the tri cyclic acid cycle, remodelling of epithelial junctions, rho family GTPase signalling and mitochondrial dysfunction.This brief report summarises the proteomic data obtained from the study described, adds to the current repertoire of data available concerning the effects of atypical antipsychotic drugs on the brain and sheds light on their biological mechanisms.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_03:47:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLorna Farrelly
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-09-24 06:36:43ID requested
12014-05-13 03:23:13announced
22014-07-24 03:47:56announcedUpdated project metadata.
Publication List
Farrelly LA, Dicker P, Wynne K, English J, Cagney G, F, รถ, cking M, Cotter DR, Adolescent Risperidone treatment alters protein expression associated with protein trafficking and cellular metabolism in the adult rat prefrontal cortex. Proteomics, 14(12):1574-8(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Rat, prefrontal, cortex, antipsychotic, LC-MSMS
Contact List
Lorna Farrelly
contact affiliationRCSI
contact emaillornafarrelly@rcsi.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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