PXD000475

PXD000475 is an original dataset announced via ProteomeXchange.

Title	Yersinia enterocolitica SOR17
Description	We work with the bacterium Yersinia enterocolitica, a gastrointestinal pathogen. In this study we characterized a bacterial mutant strain called SOR17. We compared the protein extracts of SOR17 with that of the parental strain JB580v. Bacteria were grown in triplicate for 5 h at 37°C, and following bacterial disruption by French press, proteins were fractionated into soluble or membrane proteins. We also compared total protein extracts of bacteria grown for 16 h at 27°C. Two-dimensional electrophoresis were performed and proteins were stained with Coomassie brilliant blue. Spots whose abundance changed more than 2 fold were excised and sent to mass spectrometry for identification by either MALDI-TOF PMF or LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-07-28_00:49:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000475
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ombeline Rossier
SpeciesList	scientific name: Bacteria; NCBI TaxID: 2; scientific name: Eubacteriaceae; NCBI TaxID: 186806; scientific name: Yersinia enterocolitica; NCBI TaxID: NCBITaxon:630;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; instrument model: LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-19 06:52:16	ID requested
1	2014-04-16 13:58:57	announced
⏵ 2	2014-07-28 00:49:13	announced	Updated project metadata.

Kakoschke T, Kakoschke S, Magistro G, Schubert S, Borath M, Heesemann J, Rossier O, The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica. PLoS One, 9(1):e86113(2014) [pubmed]

submitter keyword: Yersinia, enterocolitica, SOR17

Ombeline Rossier
contact affiliation	Max von Pettenkofer-Institut
contact email	Rossier@mvp.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000475
     1. Label: PRIDE project
     2. Name: Yersinia enterocolitica SOR17