PXD000474

PXD000474 is an original dataset announced via ProteomeXchange.

Title	Simulated phosphopeptide spectral library for confident site localization
Description	We have developed a new workflow to unambiguously localize phosphorylation sites on proteins. We demonstrate that spectral matching of phosphopeptide datasets against a library of the well-simulated spectra provided higher sensitivity for confident site localization than other tested programs. To computationally simulate tandem mass spectra representing all possible singly phosphorylated forms of a peptide, characteristic fragment ions are predicted from ions of their dephosphorylated form generated by beam-type collision-induced dissociation.
HostingRepository	PRIDE
AnnounceDate	2015-03-17
AnnouncementXML	Submission_2015-03-17_08:06:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susumu Imanishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-19 01:29:03	ID requested
⏵ 1	2015-03-17 08:06:54	announced

Suni V, Imanishi SY, Maiolica A, Aebersold R, Corthals GL, Confident site localization using a simulated phosphopeptide spectral library. J Proteome Res, 14(5):2348-59(2015) [pubmed]

curator keyword: Biomedical, Technical

submitter keyword: Simulated spectral library, phosphopeptide, HeLa, synthetic peptide, LC-MS/MS

Garry L. Corthals
contact affiliation	Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Finland
contact email	garry.corthals@btk.fi
lab head
Susumu Imanishi
contact affiliation	BTK
contact email	susumu.imanishi@btk.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000474
     1. Label: PRIDE project
     2. Name: Simulated phosphopeptide spectral library for confident site localization