PXD000472 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines |
Description | The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition were done. Peaks lists were generated with Mascot Distiller (version 2.3) in MGF format and Database searches were with Mascot Server (version 2.3) against the SwissProt database restricted to human sequences (release October 2012) and trypsin cleavage. Restrictions were 7ppm for parent ions and 0.8 Da for fragment masses. Allowed modifications were phosphorylation of Ser/Thr/Tyr, pyro-Glu (N-term) and methionine oxidation and one miss-cleavage allowed. Quantification was by label-free using peak heights of extracted ion chromatograms (XICs) constructed with narrow mass windows (7ppm) and time windows (1.5 minutes). Pescal, a computer program written in house, was used to automate the generation of XICs and to calculate peak heights. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-28 |
AnnouncementXML | Submission_2014-07-28_00:48:46.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000472 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Pedro Casado-Izquierdo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue; deaminated residue |
Instrument | LTQ Orbitrap; instrument model: LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-09-18 01:14:17 | ID requested | |
1 | 2014-04-11 06:48:18 | announced | |
⏵ 2 | 2014-07-28 00:48:47 | announced | Updated project metadata. |
Publication List
Casado P, Bilanges B, Rajeeve V, Vanhaesebroeck B, Cutillas PR, Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in MCF7 breast cancer cells. Mol Cell Proteomics, 13(3):836-48(2014) [pubmed] |
Keyword List
submitter keyword: MCF7, Room Temperature exposition, TiO2 Phosphoenrichment, Label Free |
Contact List
Pedro Casado-Izquierdo |
contact affiliation | Cell Signalling |
contact email | p.m.casado-izquierdo@qmul.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000472 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000472
- Label: PRIDE project
- Name: Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines