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PXD000472

PXD000472 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnvironmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines
DescriptionThe breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition were done. Peaks lists were generated with Mascot Distiller (version 2.3) in MGF format and Database searches were with Mascot Server (version 2.3) against the SwissProt database restricted to human sequences (release October 2012) and trypsin cleavage. Restrictions were 7ppm for parent ions and 0.8 Da for fragment masses. Allowed modifications were phosphorylation of Ser/Thr/Tyr, pyro-Glu (N-term) and methionine oxidation and one miss-cleavage allowed. Quantification was by label-free using peak heights of extracted ion chromatograms (XICs) constructed with narrow mass windows (7ppm) and time windows (1.5 minutes). Pescal, a computer program written in house, was used to automate the generation of XICs and to calculate peak heights.
HostingRepositoryPRIDE
AnnounceDate2014-07-28
AnnouncementXMLSubmission_2014-07-28_00:48:46.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000472
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPedro Casado-Izquierdo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue; deaminated residue
InstrumentLTQ Orbitrap; instrument model: LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-09-18 01:14:17ID requested
12014-04-11 06:48:18announced
22014-07-28 00:48:47announcedUpdated project metadata.
Publication List
Casado P, Bilanges B, Rajeeve V, Vanhaesebroeck B, Cutillas PR, Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in MCF7 breast cancer cells. Mol Cell Proteomics, 13(3):836-48(2014) [pubmed]
Keyword List
submitter keyword: MCF7, Room Temperature exposition, TiO2 Phosphoenrichment, Label Free
Contact List
Pedro Casado-Izquierdo
contact affiliationCell Signalling
contact emailp.m.casado-izquierdo@qmul.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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