PXD000471 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling |
Description | Supplemental data for the article: Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling Eunkyung An, Manikandan Narayanan, and Aleksandra Nita-Lazar* *corresponding author: Cellular Networks Proteomics Unit Laboratory of Systems Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda, Maryland, 20892, USA Tel. +1 301-451-4394 Fax: +1 301-480-5170 E-mail: nitalazarau@niaid.nih.gov This dataset includes: 1. Raw LC-MS(/MS) spectra (*.raw), and 2. The output from data analyses using IP2 (Intergrated Proteomics Application, San Diego, CA) searched against the UniProt_mouse_01-18-2011 set of protein sequences (normal + reversed). Note that the version of IP2 that was used could only analyze two SILAC channels at a time, so two analyses were performed (light-medium, light-heavy) (the current version can analyze 3-plex SILAC all together in one analysis). Also, IP2 was run using two criteria (1 or 2 peptides per protein). Lanes D and E were lanes of an SDS-PAGE gel, and each was 3-plex SILAC: Lane D: Light (Osteoclast Precursor), Medium (Mature Osteoclast), Heavy (Intermediate Osteoclast) Lane E: Light (Osteoclast Precursor), Medium (Intermediate Osteoclast), Heavy (Mature Osteoclast) |
HostingRepository | PRIDE |
AnnounceDate | 2014-12-02 |
AnnouncementXML | Submission_2014-12-02_00:31:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aleksandra Nita-Lazar |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | 6x(13)C: 4x(15)N labeled L-arginine; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine; (4: 4 |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-09-18 00:54:31 | ID requested | |
1 | 2014-07-22 05:42:38 | announced | |
2 | 2014-07-25 01:38:48 | announced | Updated project metadata. |
⏵ 3 | 2014-12-02 00:31:04 | announced | Updated project metadata. |
Publication List
An E, Narayanan M, Manes NP, Nita-Lazar A, Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling. Mol Cell Proteomics, 13(10):2687-704(2014) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: mouse, osteoclast, triple SILAC, LC-MSMS |
Contact List
Aleksandra Nita-Lazar |
contact affiliation | NIAID |
contact email | nitalazarau@niaid.nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000471 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000471
- Label: PRIDE project
- Name: Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling