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PXD000471

PXD000471 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling
DescriptionSupplemental data for the article: Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling Eunkyung An, Manikandan Narayanan, and Aleksandra Nita-Lazar* *corresponding author: Cellular Networks Proteomics Unit Laboratory of Systems Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda, Maryland, 20892, USA Tel. +1 301-451-4394 Fax: +1 301-480-5170 E-mail: nitalazarau@niaid.nih.gov This dataset includes: 1. Raw LC-MS(/MS) spectra (*.raw), and 2. The output from data analyses using IP2 (Intergrated Proteomics Application, San Diego, CA) searched against the UniProt_mouse_01-18-2011 set of protein sequences (normal + reversed). Note that the version of IP2 that was used could only analyze two SILAC channels at a time, so two analyses were performed (light-medium, light-heavy) (the current version can analyze 3-plex SILAC all together in one analysis). Also, IP2 was run using two criteria (1 or 2 peptides per protein). Lanes D and E were lanes of an SDS-PAGE gel, and each was 3-plex SILAC: Lane D: Light (Osteoclast Precursor), Medium (Mature Osteoclast), Heavy (Intermediate Osteoclast) Lane E: Light (Osteoclast Precursor), Medium (Intermediate Osteoclast), Heavy (Mature Osteoclast)
HostingRepositoryPRIDE
AnnounceDate2014-12-02
AnnouncementXMLSubmission_2014-12-02_00:31:03.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAleksandra Nita-Lazar
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList6x(13)C: 4x(15)N labeled L-arginine; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine; (4: 4
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-09-18 00:54:31ID requested
12014-07-22 05:42:38announced
22014-07-25 01:38:48announcedUpdated project metadata.
32014-12-02 00:31:04announcedUpdated project metadata.
Publication List
An E, Narayanan M, Manes NP, Nita-Lazar A, Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling. Mol Cell Proteomics, 13(10):2687-704(2014) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: mouse, osteoclast, triple SILAC, LC-MSMS
Contact List
Aleksandra Nita-Lazar
contact affiliationNIAID
contact emailnitalazarau@niaid.nih.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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