PXD000467

PXD000467 is an original dataset announced via ProteomeXchange.

Title	Endogenous S-nitrosoproteome in colorectal cancer patients
Description	Total lysate of tumorous and adjacent normal tissues from each colorectal cancer patient are followed by S-alkylating biotin switch strategy. All biotinylated peptides are analyzed by label-free quantitation and LC-MS/MS analysis. All peak lists from 66 raw MS/MS spectra were processed to mgf format by Mascot Distiller v2.1.1.0. The datasets were batch-searched and combined searched by Mascor v2.2 against the Swissprot v 57.8 human database. PEO-iodoacetyl-biotin (C, +414 Da), Caebamidomethyl (C) and oxidation (M) were specified as variable modifications. Peptides were considered to be identified if the Mascot individual ion score was higher than the Mascot identity score (p less than 0.05). To evaluate the false discovery rate in protein identification, a decoy database search against a randomized decoy database created by Mascot using identical search parameters and validation criteria were also performed. The search results in Mascot were exported in eXtensive Markup Language (XML) data format.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:44:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Ju Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-17 01:58:21	ID requested
1	2014-08-20 04:23:40	announced
2	2014-08-21 00:30:24	announced	Updated project metadata.
⏵ 3	2014-10-23 02:44:14	announced	Updated project metadata.

Chen YJ, Ching WC, Chen JS, Lee TY, Lu CT, Chou HC, Lin PY, Khoo KH, Chen JH, Chen YJ, Decoding the s-nitrosoproteomic atlas in individualized human colorectal cancer tissues using a label-free quantitation strategy. J Proteome Res, 13(11):4942-58(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: human, S-nitrosylation, biotin switch, colorectal cancer, tissue

Yi-Ju Chen
contact affiliation	Academia Sinica
contact email	tp7249@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000467

PRIDE project URI

• PRIDE
  1. PXD000467
     1. Label: PRIDE project
     2. Name: Endogenous S-nitrosoproteome in colorectal cancer patients