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PXD000461

PXD000461 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of telomere-binding proteins by enChIP-MS
DescriptionWe identified the telomere associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitaion (enChIP). Purified proteins were analyzed by GeLC-MS/MS. Tandem mass spectra were extracted by Proteome Discoverer 1.2. Database searchs were submitted to an in-house Mascot server version 2.4.1. Mascot was set up to search the Swissprot database assuming the digestion enzyme as trypsin. Carbamidomethylation of cysteine was set as a fixed modification. Oxidized methionine, acetylation of N terminus and conversion of glutamine to Pyro-Glu at N terminus were set as variable modifications. The precursor mass tolerances were 10 ppm and the tolerance of the MS/MS ions was 0.8 Da. In Scaffold 3.4.5, database search results were grouped according to gel bands.
HostingRepositoryPRIDE
AnnounceDate2014-08-08
AnnouncementXMLSubmission_2014-08-08_00:31:23.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000461
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKazunobu Saito
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deaminated residue
InstrumentLTQ Orbitrap Velos; instrument model: LTQ Orbitrap velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-09-12 04:04:20ID requested
12013-11-12 03:36:17announced
12013-11-12 04:31:37announced
22014-07-28 00:48:10announcedUpdated project metadata.
32014-08-08 00:31:23announcedUpdated project metadata.
Publication List
Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H, Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep, 3():3171(2013) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: mouse, Telomere binding protein, LC-MS/MS
Contact List
Kazunobu Saito
contact affiliationResearch Institute for Microbial Diseases
contact emailkazusa@biken.osaka-u.ac.jp
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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