PXD000459

PXD000459 is an original dataset announced via ProteomeXchange.

Title	Identification of SUMO sites by LC-MSMS
Description	We developed a novel method for the identification of SUMO sites by expression of His-tagged SUMO mutants and affinity purification of SUMOylated proteins, followed by trypsin digestion and immunocapture of peptides containing diglycine signature tags. Lab Head: Dr Pascale Cossart, pascale.cossart@pasteur.fr Institut Pasteur Unité des Interactions Bactéries-Cellules, Inserm U604, INRA USC2020 25 rue du Dr. Roux 75015 Paris France
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:21:13.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis Impens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-11 06:03:51	ID requested
1	2014-07-29 02:59:25	announced
2	2014-07-30 01:14:54	announced	Updated project metadata.
3	2014-09-10 07:35:04	announced	Updated project metadata.
⏵ 4	2024-10-22 04:21:16	announced	2024-10-22: Updated project metadata.

10.1073/pnas.1413825111;

Impens F, Radoshevich L, Cossart P, Ribet D, Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli. Proc Natl Acad Sci U S A, 111(34):12432-7(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: LC-MSMS, HeLa cells,SUMO, SILAC, immunocapture

Francis Impens
contact affiliation	Institut Pasteur
contact email	francis.impens@pasteur.fr
lab head
Francis Impens
contact affiliation	Institut Pasteur
contact email	francis.impens@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000459

PRIDE project URI

• PRIDE
  1. PXD000459
     1. Label: PRIDE project
     2. Name: Identification of SUMO sites by LC-MSMS