PXD000452

PXD000452 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis in Giardia duodenalis yields insights into strain virulence and antigenic variation
Description	Giardia duodenalis is a protozoan parasite of the small intestine in vertebrates, including humans. Assemblage A of G. duodenalis is one of two discrete subtypes that infects humans, and is considered a zoonotic assemblage. Two G. duodenalis Assemblage A strains BRIS/95/HEPU/2041 and BRIS/83/HEPU/106, constituting virulent and control strains respectively, were analysed in one of the first comparative shotgun proteomic studies performed in this parasite. Protein extracts were prepared using a multiplatform approach with both an in-gel and in-solution sample preparation to enable us to assess the complementarity for future Giardia proteomic studies. Protein analysis revealed that BRIS/95/HEPU/2041 possessed a wider and more varied repertoire of variant surface proteins (VSPs), which are hypothesised to be involved in host adaptation, immune evasion and virulence. A total of 38 VSPs were identified, with 3 common to strains, 6 unique to BRIS/83/HEPU/106 and 26 unique to BRIS/95/HEPU/2041. Additionally up to 25.6% of all differentially expressed proteins in BRIS/95/HEPU/2041 belonged to the VSP family, a trend not seen in the control BRIS/83/HEPU/106. Greater antigen variation in BRIS/95/HEPU/2041 may explain aspects of virulence phenotypes in G. duodenalis, with a highly diverse population capable of evading host immune responses.
HostingRepository	PRIDE
AnnounceDate	2014-10-15
AnnouncementXML	Submission_2014-10-15_00:30:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	monohydroxylated residue; carbamoylated residue; deaminated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-09 02:54:46	ID requested
1	2014-10-14 01:26:45	announced
⏵ 2	2014-10-15 00:30:19	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical, Biological

submitter keyword: VSPs, Giardia duodenalis, label-free shotgun proteomics, strain virulence

Samantha Emery
contact affiliation	Chemistry and Biomolecular Sciences
contact email	samantha.emery@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000452
     1. Label: PRIDE project
     2. Name: Proteomic analysis in Giardia duodenalis yields insights into strain virulence and antigenic variation