PXD000448

PXD000448 is an original dataset announced via ProteomeXchange.

Title	E-cadherin interactome complexity and robustness resolved by quantitative proteomics
Description	E-cadherin-mediated cell-cell adhesion and signaling plays an essential role in development and maintenance of healthy epithelial tissues. Adhesiveness mediated by E-cadherin is conferred by its extracellular cadherin domains and is regulated by an assembly of adaptors and enzymes associated with its cytoplasmic tail. Here, we used proximity biotinylation and quantitative proteomics to identify 561 proteins in the vicinity of E-cadherin’s cytoplasmic tail. Isolation of E-cadherin-mediated adhesion plaques from a cell-glass interface added positional context to the proteomic results. Moreover, using expression of GFP-tagged fusion proteins, we determine the subcellular localization of 83 of the E-cadherin proximal proteins, and identify 24 novel adherens junction components. We employed a structure-informed database of protein-protein interactions to construct a comprehensive E-cadherin interactome, containing 82 known and 419 previously uncharacterized proteins. Finally, we found that calcium chelation did not disrupt most protein interactions with E-cadherin, suggesting that most E-cadherin interactions are independent of cell-cell adhesion.
HostingRepository	PRIDE
AnnounceDate	2016-07-06
AnnouncementXML	Submission_2016-07-06_04:12:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Zanivan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-04 01:21:53	ID requested
⏵ 1	2016-07-06 04:12:58	announced

Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R, E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci Signal, 7(354):rs7(2014) [pubmed]

submitter keyword: E-cadherin

SILAC

label-free

interactome

cancer

Sara Zanivan
contact affiliation	Vascular Proteomics Lab, Cancer Research UK Beatson Institute
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Sara Zanivan
contact affiliation	Vascular Proteomics
contact email	s.zanivan@beatson.gla.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000448

PRIDE project URI

• PRIDE
  1. PXD000448
     1. Label: PRIDE project
     2. Name: E-cadherin interactome complexity and robustness resolved by quantitative proteomics