PXD000434

PXD000434 is an original dataset announced via ProteomeXchange.

Title	Erythrocyte Proteome & Terminome
Description	Proteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:49:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Lange
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 2x(13)C: 4x(2)H labeled dimethylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-08-29 01:43:05	ID requested
1	2014-02-24 03:06:56	announced
2	2014-03-05 03:49:00	announced	Updated publication reference for PubMed record(s): 24555563.
⏵ 3	2014-07-24 03:49:59	announced	Updated project metadata.

Lange PF, Huesgen PF, Nguyen K, Overall CM, -acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res, 13(4):2028-44(2014) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

submitter keyword: Human, Erythrocyte, RBC, red blood cell, terminus, N termini, acetylation

Philipp Lange
contact affiliation	Centre for Blood Research
contact email	lange.philipp@ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000434

PRIDE project URI

• PRIDE
  1. PXD000434
     1. Label: PRIDE project
     2. Name: Erythrocyte Proteome & Terminome