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PXD000434

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-07-24
  • AnnouncementXML: Submission_2014-07-24_03:49:58.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Philipp Lange
  • Title: Erythrocyte Proteome & Terminome
  • Description: Proteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 2x(13)C: 4x(2)H labeled dimethylated residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02013-08-29 01:43:05ID requested
12014-02-24 03:06:56announced
22014-03-05 03:49:00announcedUpdated publication reference for PubMed record(s): 24555563.
32014-07-24 03:49:59announcedUpdated project metadata.

Publication List

  1. Lange PF, Huesgen PF, Nguyen K, Overall CM, Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res, 13(4):2028-44(2014) [pubmed]

Keyword List

  1. ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
  2. submitter keyword: Human, Erythrocyte, RBC, red blood cell, terminus, N termini, acetylation

Contact List

    Philipp Lange
    • contact affiliation: Centre for Blood Research
    • contact email: lange.philipp@ubc.ca
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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