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PXD000434

PXD000434 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleErythrocyte Proteome & Terminome
DescriptionProteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_03:49:58.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilipp Lange
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 2x(13)C: 4x(2)H labeled dimethylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-08-29 01:43:05ID requested
12014-02-24 03:06:56announced
22014-03-05 03:49:00announcedUpdated publication reference for PubMed record(s): 24555563.
32014-07-24 03:49:59announcedUpdated project metadata.
Publication List
Lange PF, Huesgen PF, Nguyen K, Overall CM, Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res, 13(4):2028-44(2014) [pubmed]
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
submitter keyword: Human, Erythrocyte, RBC, red blood cell, terminus, N termini, acetylation
Contact List
Philipp Lange
contact affiliationCentre for Blood Research
contact emaillange.philipp@ubc.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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