PXD000430

PXD000430 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	in Vitro Brominated Peptides Spiked into U373 cell digestion
Description	We performed nanoLC-MS/MS analysis of an in vitro generated, trypsin-digested brominated human serum albumin standard, spiked into a complex trypsin-digested proteomic background, in an LTQ-Orbitrap instrument. We found that brominated peptides spiked in at a 1-10% ratio (mass:mass) were easily identified by manual inspection when higher-energy collisional dissociation (HCD) and collision induced dissociation (CID) were employed as the dissociation mode; however, confident assignment of brominated peptides from protein database searches required a novel approach. By addition of a custom modification, corresponding to the substitution of a single bromine with 81Br rather than 79Br for dibromotyrosine (79Br81BrY), the number of validated assignments for peptides containing dibromotyrosine increased significantly when analyzing both high resolution and low resolution MS/MS data.
HostingRepository	PRIDE
AnnounceDate	2013-08-28
AnnouncementXML	Submission_2013-08-28_07:59:21.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HL Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; monobrominated L-phenylalanine; 6'-bromo-L-tryptophan; brominated tyrosine; L-bromohistidine; dibromo
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status
0	2013-08-27 07:59:20	ID requested
⏵ 1	2013-08-28 07:59:21	announced
⏵ 1	2014-07-25 01:35:46	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: protein bromination
mass spectrometry
HCD
CID
post-translational modification
proteomics
bioinformatics

submitter keyword: protein bromination

mass spectrometry

HCD

CID

post-translational modification

proteomics

bioinformatics

Contact List

HL Liu
contact affiliation	Department of Pharmacology and Toxicology
contact email	hu2liu@utmb.edu

Full Dataset Link List

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Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/08/PXD000430