PXD000425

PXD000425 is an original dataset announced via ProteomeXchange.

Title	MS identification of the Cry proteins in Bacillus thuringiensis CT-43
Description	In Bacillus thuringiensis CT-43, five insecticidal crystal proteins (ICPs, Cry protein) are encoded. We extracted the Cry proteins, ran the SDS PAGE (two Cry protein bands were observed), and tried to identify the composition of the two Cry protein bands in the SDS PAGE. The bioinformatics pipeline is described as follows: First, we converted the original mass spectrum files to the mgf file (peaks file), then the mgf files were searched against the Bacillusthuringiensis CT-43 protein database using Mascot (version 2.3.02). The search parameters were: i) trypsin was chosen as the enzyme with one missed cleavage allowed; ii) the fixed modifications of carbamidomethylation were set as Cys, and variable modifications of oxidation as Met; iii) peptide tolerance was set as 0.05 Da, and MS/MS tolerance was set as 0.1 Da. The peptide charge was set as Mr, and monoisotopic mass was chosen. An automatic decoy database search strategy was employed to estimate the false discovery rate (FDR). The FDR was calculated as the false positive matches divided by the total matches. In the final search results, the FDR was less than 1.5%.
HostingRepository	PRIDE
AnnounceDate	2020-01-24
AnnouncementXML	Submission_2020-01-24_05:07:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000425
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Han Mei
SpeciesList	scientific name: Bacillus thuringiensis; NCBI TaxID: 1428;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600; instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2013-08-20 08:27:49	ID requested
⏵ 1	2020-01-24 05:07:48	announced

Wang J, Mei H, Qian H, Tang Q, Liu X, Yu Z, He J, Expression profile and regulation of spore and parasporal crystal formation-associated genes in Bacillus thuringiensis. J Proteome Res, 12(12):5487-501(2013) [pubmed]

submitter keyword: MS, Cry, Bacillus thuringiensis

Jin He
contact affiliation	State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University , No. 1 Shizishan Street, Wuhan, Hubei 430070, China
contact email	hejin@mail.hzau.edu.cn
lab head
Han Mei
contact affiliation	Huazhong Agricultural University
contact email	meihan0519@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000425
     1. Label: PRIDE project
     2. Name: MS identification of the Cry proteins in Bacillus thuringiensis CT-43