PXD000406

PXD000406 is an original dataset announced via ProteomeXchange.

Title	Candida albicans induces pro-inflammatory and anti-apoptotic signals in macrophages as revealed by quantitative proteomics and phosphoproteomics.
Description	Proteomic analysis: A stable isotope labeling approach (SILAC) was used to study changes in protein expression from murine macrophages in response to 3h of interaction with C. albicans (1:1 ratio) . Proteins were identified by Gel-LC-MS (1D SDS-PAGE, trypsin digestion and analysis by EASY-nLC LTQ Orbitrap XL Mass Spectrometer). Protein quantitation and identification was carried out with Proteome Discoverer v1.2 software. The relative abundance of a protein was measured by averaging the intensities of all peptides identified for the protein at each sample. Three independent biological replicas and two technical replicas of each were performed. Phosphoproteomic analysis: Two different and complementary phosphopeptide enrichment approaches were performed. Equal amounts of SILAC samples from control macrophages (grown in “light medium” 12C6-arginine and 12C6-lysine) and macrophages after 3h of interaction with C. albicans (grown in “heavy medium” 13C6-arginine and 13C6-lysine) were mixed and digested prior to phosphopeptide enrichment by ion metal affinity chromatography (IMAC) combined with TiO2 enrichment (SIMAC) or by Calcium Phosphate Precipitation (CPP) combined with TiO2. Three biological and 2 technical replicas of each condition were treated. All the SIMAC and CPP samples were analyzed by EASY-nLC LTQ Orbitrap XL Mass Spectrometer. Raw data files were processed with the Proteome Discoverer v1.2 software for peptide and protein identification and the quantification of phosphorylation changes. Finally, phosphorylation site ratios measured in both technical replica experiments were averaged prior to further data analysis.
HostingRepository	PRIDE
AnnounceDate	2020-01-24
AnnouncementXML	Submission_2020-01-24_05:43:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jose Antonio Reales-Calderon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-08-08 06:15:45	ID requested
⏵ 1	2020-01-24 05:43:48	announced

Reales-Calder, ó, n JA, Sylvester M, Strijbis K, Jensen ON, Nombela C, Molero G, Gil C, Candida albicans induces pro-inflammatory and anti-apoptotic signals in macrophages as revealed by quantitative proteomics and phosphoproteomics. J Proteomics, 91():106-35(2013) [pubmed]

submitter keyword: Apoptosis

Mass Spectrometry

Phosphoproteins

Quantification

SILAC

Candida albicans

Inflammation

Macrophages

Concha Gil
contact affiliation	Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact email	gloros@farm.ucm.es
lab head
Jose Antonio Reales-Calderon
contact affiliation	Microbiologia II
contact email	jareales@farm.ucm.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000406
     1. Label: PRIDE project
     2. Name: Candida albicans induces pro-inflammatory and anti-apoptotic signals in macrophages as revealed by quantitative proteomics and phosphoproteomics.