Updated project metadata. This is a 2-plex SILAC-based quantitative proteomic study. It quantifies abundance changes of the apical membrane proteins of the kidney collecting duct model cells (mpkCCD) in response to vasopressin. Cells were labeled with normal or heavy lysine and arginine and then exposed to vehicle or the vasopressin analogy dDAVP (1 nM, 1 hour). Apical membrane proteins of vehicle and dDAVP-treated cells were enriched via surface biotinylation and affinity purification prior to LC-MS/MS analysis.