PXD000391

PXD000391 is an original dataset announced via ProteomeXchange.

Title	Salmonella secretome analysis
Description	Researchers identified effector proteins secreted into defined minimal medium designed to induce expression of the SPI-2 TTSS and its effectors. The secretome of the parent strain was compared to those of strains missing essential (ssaK::cat) or regulatory (ssaL) components of the SPI-2 TTSS. 20 known SPI-2 effectors were identified. To identify novel effector proteins, the secretome data was coupled with machine learning and 12 candidate proteins were selected for further characterization. Using CyaA reporter fusions, six novel type III effectors were identified and two additional proteins that were secreted into J774 macrophages independently of a TTSS were also identified.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:29:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Salmonella typhimurium (strain 14028s / SGSC 2262); NCBI TaxID: 588858;
ModificationList	Oxidation
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-08-01 04:13:23	ID requested
1	2013-10-07 05:47:33	announced
1	2013-10-07 08:32:34	announced
⏵ 2	2014-07-25 01:29:07	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: secretome, effector proteins, machine learning

Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/10/PXD000391

PRIDE project URI

• PRIDE
  1. PXD000391
     1. Label: PRIDE project
     2. Name: Salmonella secretome analysis