PXD000384

PXD000384 is an original dataset announced via ProteomeXchange.

Title	V5-tagged LIMD1 in HEK293 cells with or without JNK2 activation
Description	The experiment was conducted to identify JNK phosphorylation sites on human LIM domain containing protein-1 (LIMD1). V5-tagged LIMD1 was co-transfected or not with a constitutively active form of JNK2 and purified. The purified proteins were subjected to SDS-PAGE, protease K digestion and MS/MS analysis. The data were searched against Swissprot using Mascot server v2.3. The fixed modification was set to carbamidomethylation of C, and variable modifications were set to oxidation of M, phosphorylation of ST, phosphorylation of Y. Mass values were set as monoisotopic. Peptide mass tolerance was set to +/-10ppm, and MS/MS tolerance was set to +/-0.8Da.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:47:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000384
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gongping Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	instrument model: LTQ; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-31 01:20:04	ID requested
1	2014-07-09 03:03:52	announced
⏵ 2	2014-07-24 03:47:33	announced	Updated project metadata.

Sun G, Irvine KD, Ajuba family proteins link JNK to Hippo signaling. Sci Signal, 6(292):ra81(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, LC-MS/MS

Gongping Sun
contact affiliation	Waksman Institute of Microbiology
contact email	gongping@eden.rutgers.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000384

PRIDE project URI

• PRIDE
  1. PXD000384
     1. Label: PRIDE project
     2. Name: V5-tagged LIMD1 in HEK293 cells with or without JNK2 activation