PXD000377

PXD000377 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of purified protein derivative of mycobacterium tuberculosis
Description	Proteomic analysis of Connaught Tuberculin 68 (PPD-CT68), a tuberculin preparation generated froM. tuberculosis, was carried out in this study. PPD-CT68 is the protein component of a commercially available tuberculin preparation, Tubersol, which is used for tuberculin skin testing. Using a high resolution LTQ-Orbitrap Velos mass spectrometer, we identified 265 different proteins. The identified proteins were compared with those identified from PPD M. bovis, PPD M. avium and PPD-S2 from previous mass spectrometry-based studies. In all, 142 proteins were fo PPDs (i.e. proteins in either PPD-CT68 or PPD-S2), 37 proteins were found to be shared with M. avium PPD and 80 were shared with M. bovis PPD. Alignment of PPD-CT68 proteins with proteins encoded by 24 lung infecting bacteria revealed a number of similar proteins (206 bacterial proteins shared epitopes with 47 PPD-CT68 proteins), which could potentially be involved in causing cross-reactivity
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:26:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-29 05:25:28	ID requested
1	2014-01-23 02:45:39	announced
2	2014-04-16 01:36:18	announced	Updated publication reference for PubMed record(s): 23870090.
⏵ 3	2014-07-25 01:26:37	announced	Updated project metadata.

Prasad TS, Verma R, Kumar S, Nirujogi RS, Sathe GJ, Madugundu AK, Sharma J, Puttamallesh VN, Ganjiwale A, Myneedu VP, Chatterjee A, Pandey A, Harsha H, Narayana J, Proteomic analysis of purified protein derivative of Mycobacterium tuberculosis. Clin Proteomics, 10(1):8(2013) [pubmed]

submitter keyword: LC-MS/MS, CT-68

Akhilesh Pandey
contact affiliation	Institute of Genetic Medicine
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/01/PXD000377

PRIDE project URI

• PRIDE
  1. PXD000377
     1. Label: PRIDE project
     2. Name: Proteomic analysis of purified protein derivative of mycobacterium tuberculosis