<< Full experiment listing

PXD000377

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-07-25
  • AnnouncementXML: Submission_2014-07-25_01:26:36.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Akhilesh Pandey
  • Title: Proteomic analysis of purified protein derivative of mycobacterium tuberculosis
  • Description: Proteomic analysis of Connaught Tuberculin 68 (PPD-CT68), a tuberculin preparation generated froM. tuberculosis, was carried out in this study. PPD-CT68 is the protein component of a commercially available tuberculin preparation, Tubersol, which is used for tuberculin skin testing. Using a high resolution LTQ-Orbitrap Velos mass spectrometer, we identified 265 different proteins. The identified proteins were compared with those identified from PPD M. bovis, PPD M. avium and PPD-S2 from previous mass spectrometry-based studies. In all, 142 proteins were fo PPDs (i.e. proteins in either PPD-CT68 or PPD-S2), 37 proteins were found to be shared with M. avium PPD and 80 were shared with M. bovis PPD. Alignment of PPD-CT68 proteins with proteins encoded by 24 lung infecting bacteria revealed a number of similar proteins (206 bacterial proteins shared epitopes with 47 PPD-CT68 proteins), which could potentially be involved in causing cross-reactivity
  • SpeciesList: scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
  • ModificationList: iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02013-07-29 05:25:28ID requested
12014-01-23 02:45:39announced
22014-04-16 01:36:18announcedUpdated publication reference for PubMed record(s): 23870090.
32014-07-25 01:26:37announcedUpdated project metadata.

Publication List

  1. Prasad TS, Verma R, Kumar S, Nirujogi RS, Sathe GJ, Madugundu AK, Sharma J, Puttamallesh VN, Ganjiwale A, Myneedu VP, Chatterjee A, Pandey A, Harsha H, Narayana J, Proteomic analysis of purified protein derivative of Mycobacterium tuberculosis. Clin Proteomics, 10(1):8(2013) [pubmed]

Keyword List

  1. submitter keyword: LC-MS/MS, CT-68

Contact List

    Akhilesh Pandey
    • contact affiliation: Institute of Genetic Medicine
    • contact email: pandey@jhmi.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
[+]
Subscribe to receive all new ProteomeXchange announcements!
If you have a question or comment about ProteomeXchange, please contact us!