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PXD000376

PXD000376 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Multi-Omic Analysis of Human Naive CD4 T cells
DescriptionTechnological advances in genomics, epigenomics, transcriptomics and proteomics have enabled massively parallel measurements across thousands of genes and gene products. Such high-throughput technologies have been extensively used to carry out genome-wide studies particularly in the context of diseases. Nevertheless, a unified analysis of the genome, epigenome, transcriptome, and proteome of a single mammalian cell type to obtain a coherent view of the complex interplay between omes has not yet been undertaken. Here, we report the first multi-omic analysis of human primary naïve CD4+ T cells, revealing hundreds of unannotated mRNA transcripts, miRNAs, pseudogenes, and noncoding RNAs. Additionally, we carried out a comparative analysis of naïve CD4+ T cells with primary resting memory CD4+ T cells, which have provided novel insights into T cell biology. Overall, our data will serve as a baseline reference of a single pure population of cells for future systems level analysis of other defined cell populations.
HostingRepositoryPRIDE
AnnounceDate2015-11-12
AnnouncementXMLSubmission_2015-11-12_04:41:26.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAkhilesh Pandey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-07-29 02:43:51ID requested
12015-11-12 04:41:27announced
Publication List
Mitchell CJ, Getnet D, Kim MS, Manda SS, Kumar P, Huang TC, Pinto SM, Nirujogi RS, Iwasaki M, Shaw PG, Wu X, Zhong J, Chaerkady R, Marimuthu A, Muthusamy B, Sahasrabuddhe NA, Raju R, Bowman C, Danilova L, Cutler J, Kelkar DS, Drake CG, Prasad TS, Marchionni L, Murakami PN, Scott AF, Shi L, Thierry-Mieg J, Thierry-Mieg D, Irizarry R, Cope L, Ishihama Y, Wang C, Gowda H, Pandey A, ve CD4+ T cells. BMC Syst Biol, 9():75(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: LC-MSMS, Naive CD4+ T cells, Memory CD4+ T cells
Contact List
Akhilesh Pandey
contact affiliationJohns Hopkins University
contact emailpandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliationJohns Hopkins University
contact emailpandey@jhmi.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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