PXD000367

PXD000367 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 1917
Description	Escherichia coli Nissle 1917 (EcN) is a probiotic used for treatment of intestinal disorders. EcN improves gastrointestinal homeostasis and microbiota balance; however little is known about how this probiotic delivers effector molecules to the host. Outer membrane vesicles (OMVs) are constitutively produced by gram-negative bacteria and have a relevant role in bacteria-host interactions. Here we performed proteomic analysis of EcN OMVs. Using 1D SDSD-PAGE and highly sensitive LC-MS/MS analysis we identified 192 EcN vesicular proteins with high confidence in three independent experiments. Of these proteins, 18 were encoded by strain-linked genes and 57 were common to pathogen-derived OMVs. These proteins may contribute to the ability of this probiotic to colonize the human gut as they fulfil functions related to adhesion to host tissues, immune modulation or bacterial survival in host niches. This study describes the first global OMV proteome of a probiotic strain and provides evidence that probiotic-derived OMVs contain proteins that can target these vesicles to the host and mediate their beneficial effects on intestinal function.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_00:30:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eliandre de Oliveira
SpeciesList	scientific name: Escherichia coli Nissle 1917; NCBI TaxID: 316435;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-26 08:18:08	ID requested
1	2013-12-19 02:54:58	announced
1	2013-12-19 02:56:45	announced
2	2014-07-25 01:26:24	announced	Updated project metadata.
⏵ 3	2014-08-08 00:30:58	announced	Updated project metadata.

Aguilera L, Toloza L, Gim, é, nez R, Odena A, Oliveira E, Aguilar J, Badia J, Baldom, à L, Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 1917. Proteomics, 14(2-3):222-9(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Outer membrane vesicles, probiotics, Escherichia coli Nissle 1917

Eliandre de Oliveira
contact affiliation	Proteomics Platform
contact email	eoliveira@pcb.ub.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/12/PXD000367

PRIDE project URI

• PRIDE
  1. PXD000367
     1. Label: PRIDE project
     2. Name: Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 1917