PXD000357 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of ERK1 direct substrates using stable isotope labeled kinase assay linked phosphoproteomics |
Description | Following the kinase assay linked phosphoproteomics strategy (KALIP) (Xue, L et al., 2012), we used extracellular signal-regulated kinases 1 (ERK1) to phosphorylate the HEK293 cell lysate under the in vitro kinase assay condition. The phosphorylated proteins were then isolated and identified by mass spectrometry. The in vitro phosphorylated proteins with new phosphates were further overlapped with reported in vivo ERK1-dependent phosphoproteomics data for the identification of bona fide direct substrates of ERK1. In total, we identified 27 direct substrates of ERK1. Data analysis procedure: Raw MS files from the LTQ-Orbitrap-Velos were analyzed by Proteome Discoverer 1.3. MS/MS spectra were searched against the IPI-human database (version 3.83) containing both forward and reverse protein sequences by the SEQUEST search engine. The false discovery rate (FDR) was set to 0.01 on the peptide level. Ingenuity Pathway Analysis (IPA) was applied for the functional annotation. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_03:47:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liang Xue |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-07-22 08:16:07 | ID requested | |
1 | 2014-07-17 00:52:52 | announced | |
⏵ 2 | 2014-07-24 03:47:39 | announced | Updated project metadata. |
Publication List
Xue L, Wang P, Cao P, Zhu JK, Tao WA, Identification of extracellular signal-regulated kinase 1 (ERK1) direct substrates using stable isotope labeled kinase assay-linked phosphoproteomics. Mol Cell Proteomics, 13(11):3199-210(2014) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Phosphoproteomics, Kinase substrates, ERK1 |
Contact List
Liang Xue |
contact affiliation | Biochemistry |
contact email | xue2@purdue.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000357
- Label: PRIDE project
- Name: Identification of ERK1 direct substrates using stable isotope labeled kinase assay linked phosphoproteomics