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PXD000357

PXD000357 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of ERK1 direct substrates using stable isotope labeled kinase assay linked phosphoproteomics
DescriptionFollowing the kinase assay linked phosphoproteomics strategy (KALIP) (Xue, L et al., 2012), we used extracellular signal-regulated kinases 1 (ERK1) to phosphorylate the HEK293 cell lysate under the in vitro kinase assay condition. The phosphorylated proteins were then isolated and identified by mass spectrometry. The in vitro phosphorylated proteins with new phosphates were further overlapped with reported in vivo ERK1-dependent phosphoproteomics data for the identification of bona fide direct substrates of ERK1. In total, we identified 27 direct substrates of ERK1. Data analysis procedure: Raw MS files from the LTQ-Orbitrap-Velos were analyzed by Proteome Discoverer 1.3. MS/MS spectra were searched against the IPI-human database (version 3.83) containing both forward and reverse protein sequences by the SEQUEST search engine. The false discovery rate (FDR) was set to 0.01 on the peptide level. Ingenuity Pathway Analysis (IPA) was applied for the functional annotation.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_03:47:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiang Xue
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-07-22 08:16:07ID requested
12014-07-17 00:52:52announced
22014-07-24 03:47:39announcedUpdated project metadata.
Publication List
Xue L, Wang P, Cao P, Zhu JK, Tao WA, Identification of extracellular signal-regulated kinase 1 (ERK1) direct substrates using stable isotope labeled kinase assay-linked phosphoproteomics. Mol Cell Proteomics, 13(11):3199-210(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Phosphoproteomics, Kinase substrates, ERK1
Contact List
Liang Xue
contact affiliationBiochemistry
contact emailxue2@purdue.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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