PXD000356

PXD000356 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric analysis of Podospora anserina mitochondria
Description	Here we report data of an analysis to monitor the age-related quantitative protein composition of the mitochondria of the fungal aging model Podospora anserina. The impact of senescence on mitochondrial protein composition was analyzed by LC-MS analysis. In an untargeted proteomic approach, we identified 795 proteins in samples from juvenile and senescent wild-type cultures and obtained quantitative information for 226 of these proteins by spectral counting. Despite the broad coverage of the proteome, no substantial changes linked to the aging process could be observed. LC-MS/MS-data were analyzed using the Sequest algorithm implemented in the Bioworks 3.3.1 software (Thermo scientific) for peptide identification versus a P. anserina protein database. As enzyme specificity Trypsin was selected. Two missed cleavages were permitted and the mass tolerance was set to 10 ppm. False discovery rates were estimated by the number of spectral matches to a decoy database. Acceptance criteria and filters were set to achieve a false positive rate of 5 %.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:24:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicole Plohnke
SpeciesList	scientific name: Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina); NCBI TaxID: 515849;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-22 08:15:56	ID requested
1	2014-03-05 03:39:16	announced
⏵ 2	2014-07-25 01:24:34	announced	Updated project metadata.

Plohnke N, Hamann A, Poetsch A, Osiewacz HD, R, ö, gner M, Rexroth S, Proteomic analysis of mitochondria from senescent Podospora anserina casts new light on ROS dependent aging mechanisms. Exp Gerontol, 56():13-25(2014) [pubmed]

submitter keyword: Podospora anserina, mitochondria, LC-MS/MS, aging

Nicole Plohnke
contact affiliation	Ruhr Universität Bochum
contact email	Nicole.Plohnke@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/03/PXD000356

PRIDE project URI

• PRIDE
  1. PXD000356
     1. Label: PRIDE project
     2. Name: Mass spectrometric analysis of Podospora anserina mitochondria