PXD000352

PXD000352 is an original dataset announced via ProteomeXchange.

Title	Proteomic of spearmint (Mentha spicata L.) leaf trichomes
Description	Peltate glandular trichomes from /Mentha spicata /were purified on a Percoll gradient and soluble and membrane proteins were trypsinized and the peptides separated by nano-LC fractionation and analyzed by MALDI-MS/MS. The vast majority of the 1,666 proteins identified was housekeeping proteins or involved in primary metabolism. However, 57 were predicted to be involved in secondary metabolism. Of these, 21 were involved in the synthesis of phenylpropanoids and phenolics and 32 in terpenoid synthesis. Of the 14 membrane transporters identified, the 11 ATP-binding cassette transporters provide good material for assessing whether active transport is required for the transfer of monoterpenoid intermediates between cellular compartments and for the secretion of the final products into the subcuticular storage cavity. In conclusion, this proteome analysis of /M. spicata/ peltate trichomes has identified several candidate proteins that might be involved in terpenoid synthesis and transport. MALDI-MS/MS analyses were performed as described previously (Szopinska et al., 2011). MS data from the soluble and microsomal fractions were pooled and analyzed as a single data set using ProteinPilotTM software (AB SCIEX, v.4.0.8085). Protein identification was based on ParagonTM algorithm v.4.0 and used the Viridiplantae part of the whole NCBInr protein database (downloaded on April 14th, 2013). This algorithm in ProteinPilot was used with “identification” as the sample type, “MMTS” as cysteine modification, “4800 TOF/TOF” as the instrument, and the "Thorough" preset search setting. All reported proteins were identified with 95% or greater confidence, as determined by ProteinPilot unused scores (greater than 1.3) using the most stringent threshold of false positive discovery rate, i.e. below 10%. Protein grouping performed by ProteinPilotTM removed redundant hits.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_01:54:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Champagne
SpeciesList	scientific name: Mentha spicata (Spearmint); NCBI TaxID: 29719;
ModificationList	L-homoarginine
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-22 03:09:38	ID requested
1	2013-10-17 07:01:28	announced
1	2013-10-17 07:04:30	announced
2	2014-07-25 01:24:15	announced	Updated project metadata.
⏵ 3	2014-08-08 01:54:01	announced	Updated project metadata.

Champagne A, Boutry M, Proteomic snapshot of spearmint (Mentha spicata L.) leaf trichomes: a genuine terpenoid factory. Proteomics, 13(22):3327-32(2013) [pubmed]

curator keyword: Biological

submitter keyword: Mentha spicata, Proteomics, Secondary metabolism, Terpenoids, Glandular trichome.

Antoine Champagne
contact affiliation	Life Science Institute
contact email	antoine.champagne@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/10/PXD000352

PRIDE project URI

• PRIDE
  1. PXD000352
     1. Label: PRIDE project
     2. Name: Proteomic of spearmint (Mentha spicata L.) leaf trichomes