PXD000350

PXD000350 is an original dataset announced via ProteomeXchange.

Title	Sumoylated hnrnpa2b1 controls the sorting of miRNAs into two exosomes through binding to specific motifs
Description	Exosomes are released by most cells to the extracellular environment, and are involved in cell-to-cell-communication. Exosomes contain specific repertoires of mRNAs, miRNAs and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe short sequence motifs present in miRNAs that control their localization into exosomes. The protein hnRNPA2B1 specifically binds exosomal miRNAs through the recognition of these motifs and controls their loading into exosomes. Moreover, hnRNPA2B1 in exosomes is sumoylated, and sumoylation controls the binding of hnRNPA2B1 to miRNAs. The loading of miRNAs into exosomes can be modulated by mutagenesis of the identified motifs or changes in hnRNPA2B1 expression levels. These findings identify hnRNPA2B1 as a key player in miRNA sorting into exosomes and provide potential tools for the packaging of selected regulatory RNAs into exosomes and their use in biomedical applications.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:23:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Trevisan-Herraz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfone; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-19 03:11:11	ID requested
1	2014-04-16 13:02:48	announced
⏵ 2	2014-07-25 01:23:49	announced	Updated project metadata.

Villarroya-Beltri C, Guti, é, rrez-V, á, zquez C, S, á, nchez-Cabo F, P, é, rez-Hern, á, ndez D, V, á, zquez J, Martin-Cofreces N, Martinez-Herrera DJ, Pascual-Montano A, Mittelbrunn M, S, á, nchez-Madrid F, Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs. Nat Commun, 4():2980(2013) [pubmed]

submitter keyword: exosomes, microRNAs, hnRNPA2B1, sumoylation

Marco Trevisan-Herraz
contact affiliation	Vascular Biology and Inflammation
contact email	mtrevisan@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000350

PRIDE project URI

• PRIDE
  1. PXD000350
     1. Label: PRIDE project
     2. Name: Sumoylated hnrnpa2b1 controls the sorting of miRNAs into two exosomes through binding to specific motifs