PXD000341

PXD000341 is an original dataset announced via ProteomeXchange.

Title	Site-specific quantitation of TCR-induced phosphorylation in Jurkat T cell lines
Description	The LC-MSMS data correspond to an investigation where we compared global dynamics of TCR signaling in Jurkat cell lines with or without LAT (Linker for activation of T cells). We used triple SILAC labelling. In each experiment two series of time points activation were generated with a common time point that we used to consolidated the two separate time series. The protein extracts in each time series were digested by trypsine and the peptide submitted to SCX fractionation and TiO2 enrichment of phosphopeptides. Each sample was injected 2-3 times.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:22:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mogjib SALEK
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-15 00:44:13	ID requested
1	2013-11-15 03:22:22	announced
1	2013-11-15 03:28:19	announced
⏵ 2	2014-07-25 01:22:38	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: Phosphoproteomics, T cell, Jurakts, LAT

Mogjib SALEK
contact affiliation	Sir William Dunn School of Pathology, Oxford (UK), and Proteomics Consulting, Toulouse, France.
contact email	mogjibs@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/11/PXD000341

PRIDE project URI

• PRIDE
  1. PXD000341
     1. Label: PRIDE project
     2. Name: Site-specific quantitation of TCR-induced phosphorylation in Jurkat T cell lines