PXD000330

PXD000330 is an original dataset announced via ProteomeXchange.

Title	S. aureus COL anaerobiosis absolute protein quantification
Description	Absolute protein quantification was applied to follow the dynamics of the cytoplasmic proteome of Staphylococcus aureus in response to long-term oxygen starvation. For 1,168 proteins, the majority of all expressed proteins, molecule numbers per cell have been determined to monitor the cellular investments in single branches of bacterial life for the first time. In the presence of glucose the anaerobic protein pattern is characterized by increased amounts of glycolytic and fermentative enzymes such as Eno, GapA1, Ldh1, and PflB. Interestingly, the ferritin-like protein FtnA belongs to the most abundant proteins during anaerobic growth. Depletion of glucose finally leads to an accumulation of different enzymes such as ArcB1, ArcB2, and ArcC2 involved in arginine deiminase pathway. Concentrations of 29 exo- and 78 endometabolites were comparatively assessed and have been integrated to the metabolic networks. Here we provide an almost complete picture on the response to oxygen starvation, from signal transduction pathways to gene expression pattern, from metabolic reorganization after oxygen depletion to beginning cell death and lysis after glucose exhaustion. This experimental approach can be considered as a proof of principle how to combine cell physiology with quantitative proteomics for a new dimension in understanding simple life processes as an entity.
HostingRepository	PRIDE
AnnounceDate	2016-07-11
AnnouncementXML	Submission_2016-07-11_08:49:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Staphylococcus aureus (strain COL); NCBI TaxID: 93062;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-09 02:32:28	ID requested
⏵ 1	2016-07-11 08:49:20	announced

Z, ü, hlke D, D, ö, rries K, Bernhardt J, Maa, ß S, Muntel J, Liebscher V, Pan, é, -Farr, é J, Riedel K, Lalk M, V, ö, lker U, Engelmann S, Becher D, Fuchs S, Hecker M, Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep, 6():28172(2016) [pubmed]

curator keyword: Biological

submitter keyword: Staphylococcus aureus, anaerbiosis, absolute protein quantification, LC-MSe, LC-IMSe, Synapt

Michael Hecker
contact affiliation	Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Strasse 15, 17489 Greifswald, Germany
contact email	hecker@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000330

PRIDE project URI

• PRIDE
  1. PXD000330
     1. Label: PRIDE project
     2. Name: S. aureus COL anaerobiosis absolute protein quantification