PXD000329

PXD000329 is an original dataset announced via ProteomeXchange.

Title	Chemical profiling of live cell temporal redox dynamics in a photoautotrophic cyanobacterium
Description	We have developed a quantitative chemical probe approach for live cell labeling of proteins that are sensitive to redox modifications. We utilize this in vivo strategy coupled to mass spectrometry-based proteomics to identify 176 proteins undergoing ~5-10 fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium, Synechococcus sp. PCC 7002. This method enables the identification of redox changes in as little as 30 seconds after nutrient perturbation, and oscillations in reduction and oxidation for 60 minutes following the perturbation. The redox changes were validated by demonstrating that protein abundances did not change per global proteomic analyses. Peptides identified by MS for global and probe-labeled samples were required to be at least six amino acids in length having a mass spectra generating function score of <=1E-10, which corresponds to an FDR of <1%. Additionally, only peptides unique in identifying a single protein were utilized to estimate protein abundances, and proteins represented by <2 unique peptides were removed. This resulted in the identification of 176 redox probe labeled protein identifications, and 808 protein identifications in the global data.
HostingRepository	PRIDE
AnnounceDate	2015-08-24
AnnouncementXML	Submission_2015-08-24_01:44:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000329
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum); NCBI TaxID: 32049;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-09 02:03:32	ID requested
⏵ 1	2015-08-24 01:44:26	announced

Sadler NC, Melnicki MR, Serres MH, Merkley ED, Chrisler WB, Hill EA, Romine MF, Kim S, Zink EM, Datta S, Smith RD, Beliaev AS, Konopka A, Wright AT, Live cell chemical profiling of temporal redox dynamics in a photoautotrophic cyanobacterium. ACS Chem Biol, 9(1):291-300(2014) [pubmed]

submitter keyword: Biofuels, chemical profiling, cyanobacteria, photosynthesis, redox

Aaron T. Wright
contact affiliation	Pacific Northwest National Laboratory, USA
contact email	Aaron.Wright@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Biological Separations & Mass Spectrometry
contact email	matthew.monroe@pnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000329
     1. Label: PRIDE project
     2. Name: Chemical profiling of live cell temporal redox dynamics in a photoautotrophic cyanobacterium