PXD000326

PXD000326 is an original dataset announced via ProteomeXchange.

Title	A multi-omic analysis of an Enterococcus faecium mutant reveals genetic mutation and dramatic changes of mRNA and protein expressions
Description	Three iTRAQ8plex experiment replicate using TripleToF 5600 system. Protein identification were performed using the ProteinPilot software (version 4.0.8085,Paragon Algorithm version 4.0.0.0).All MS/MS spectra was search against amino acids sequences which is annotated from genome. The search parameters was set as follows: Cys. Alkylation: Iodoacetic acid, Digestion: Trypsin, ID Focus: Biological modifications, Search Effort: Thorough. The search results was filter using Local FDR 5%.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:20:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu liu
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationList	deamidated residue; monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-05 09:08:40	ID requested
1	2014-04-16 13:15:46	announced
⏵ 2	2014-07-25 01:20:32	announced	Updated project metadata.

Chang D, Zhu Y, An L, Liu J, Su L, Guo Y, Chen Z, Wang Y, Wang L, Wang J, Li T, Fang X, Fang C, Yang R, Liu C, A multi-omic analysis of an Enterococcus faecium mutant reveals specific genetic mutations and dramatic changes in mRNA and protein expression. BMC Microbiol, 13():304(2013) [pubmed]

submitter keyword: Enterococcus faecium,Protein,transcript,LC-MS/MS,Space flight

Liu liu
contact affiliation	Institute
contact email	jiansha-111@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000326

PRIDE project URI

• PRIDE
  1. PXD000326
     1. Label: PRIDE project
     2. Name: A multi-omic analysis of an Enterococcus faecium mutant reveals genetic mutation and dramatic changes of mRNA and protein expressions