PXD000313 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics study on rice embryo during embryogenesis by using isobaric tags for relative and absolute quantification (iTRAQ) |
Description | Sample Info.: There are five embryogenesis stages, 6, 12, 18, 24 and 30 days after pollination (DAP). We collect the rice embryos from above five stages. Protein extraction and analysis: Embryos of the five sampling time points were used for protein extraction and One hundred micro gram extracted proteins of each sampling time point was tryptic digested and then labeled with iTRAQ 113, 115, 117, 119 and 121 for 6, 12, 18, 24 and 30 DAP resceptively. The labeled peptides were mixed together and then fractionated via SCX column to 10 fraction (named from 1 to 10). Two microgram peptides of each fraction was loaded onto a nano HPLC mounted with C 18 column and tandermed with a Q-Exactive mass spectrometer. For each fraction, three technical analysis were conducted (e.g., for fraction 5, the three injection raw files were named as 5_1, 5_2 and 5_3). Protein search: Each group of raw files (e.g., 1_1, 2_1, ... , 10_1) were combined and used for Mascot search and the search results were also uploaded in this system. F009646, F009647 and F009648 represent the mascot search result of replication 1, 2 and 3 respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-25 |
AnnouncementXML | Submission_2014-07-25_01:17:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jin Zi |
SpeciesList | scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; |
ModificationList | deamidated residue; 2-pyrrolidone-5-carboxylic acid (Glu); iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; Oxidation: Methionine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-07-02 00:56:17 | ID requested | |
1 | 2013-08-06 01:44:11 | announced | |
1 | 2013-10-03 00:16:49 | announced | |
⏵ 2 | 2014-07-25 01:17:52 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Rice, Embryogenesis, iTRAQ |
Contact List
Jin Zi |
contact affiliation | Proteomics Division |
contact email | zij@genomics.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/08/PXD000313 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000313
- Label: PRIDE project
- Name: Quantitative proteomics study on rice embryo during embryogenesis by using isobaric tags for relative and absolute quantification (iTRAQ)