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PXD000313

PXD000313 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics study on rice embryo during embryogenesis by using isobaric tags for relative and absolute quantification (iTRAQ)
DescriptionSample Info.: There are five embryogenesis stages, 6, 12, 18, 24 and 30 days after pollination (DAP). We collect the rice embryos from above five stages. Protein extraction and analysis: Embryos of the five sampling time points were used for protein extraction and One hundred micro gram extracted proteins of each sampling time point was tryptic digested and then labeled with iTRAQ 113, 115, 117, 119 and 121 for 6, 12, 18, 24 and 30 DAP resceptively. The labeled peptides were mixed together and then fractionated via SCX column to 10 fraction (named from 1 to 10). Two microgram peptides of each fraction was loaded onto a nano HPLC mounted with C 18 column and tandermed with a Q-Exactive mass spectrometer. For each fraction, three technical analysis were conducted (e.g., for fraction 5, the three injection raw files were named as 5_1, 5_2 and 5_3). Protein search: Each group of raw files (e.g., 1_1, 2_1, ... , 10_1) were combined and used for Mascot search and the search results were also uploaded in this system. F009646, F009647 and F009648 represent the mascot search result of replication 1, 2 and 3 respectively.
HostingRepositoryPRIDE
AnnounceDate2014-07-25
AnnouncementXMLSubmission_2014-07-25_01:17:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJin Zi
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationListdeamidated residue; 2-pyrrolidone-5-carboxylic acid (Glu); iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; Oxidation: Methionine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-07-02 00:56:17ID requested
12013-08-06 01:44:11announced
12013-10-03 00:16:49announced
22014-07-25 01:17:52announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Rice, Embryogenesis, iTRAQ
Contact List
Jin Zi
contact affiliationProteomics Division
contact emailzij@genomics.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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