PXD000312

PXD000312 is an original dataset announced via ProteomeXchange.

Title	Mercury Uniquely Alters B-Cell Protein Phosphorylation Profiles
Description	To test the hypothesis that HgCl2 acts as a phospho-tyrosine phosphatase inhibitor in B-cells, we treated the WEHI-231 cell line for 10 min with HgCl2 at 100 uM or 250 uM, okadaic acid at 100 nM or pervanadate at 25 uM and analyzed the phosphoproteome by TiO2 affinity chromatography and LC-MS/MS. WEHI-231 cell extracts were trypsinized and phosphopeptides were isolated using TiO2 affinity chromatography. Eluates were dried and resuspended in reversed-phase HPLC loading buffer for nano-flow HPLC, electrospray ionization and analysis on a Thermo LTQ mass spectrometer. Data were analyzed using the peptide prophet algorithm in Scaffold using Mascot and X!Tandem search results as input.
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-07-28_00:40:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000312
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicholas Carruthers
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue; deaminated residue
Instrument	instrument model: LTQ; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-01 06:28:25	ID requested
1	2013-12-10 00:54:10	announced
1	2013-12-10 01:12:39	announced
⏵ 2	2014-07-28 00:40:26	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: mercury, Hg, phosphoprotein, phosphoylation, B-cell, pervanadate

Nicholas Carruthers
contact affiliation	Wayne State University
contact email	carruthers@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000312
     1. Label: PRIDE project
     2. Name: Mercury Uniquely Alters B-Cell Protein Phosphorylation Profiles