PXD000310

PXD000310 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome profiling of Caenorhabditis elegans pept-1(lg601) and N2(Bristol) during ontogenesis.
Description	PEPT-1 is responsible for the uptake of di-and tripeptides form the intestinal lumen into the epithelial cells. Knock-out of pept-1 in C.elegans results in a severe phenotype. Despite a reduction of brood size and a retarded development, pept-1(lg601) depicts increased stress resistance. Therefore, populations of pept1(lg601) as well as N2(Bristol) were synchronized and sampled at various time points during development. Applying 15N metabolic labeling in combination with shotgun LC/MS-MS, we determined the proteome of both strains during development.
HostingRepository	PRIDE
AnnounceDate	2016-07-16
AnnouncementXML	Submission_2016-07-16_00:30:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mayer
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-01 05:19:21	ID requested
1	2016-07-15 00:52:51	announced
⏵ 2	2016-07-16 00:30:22	announced	Updated project metadata.

Geillinger KE, Kuhlmann K, Eisenacher M, Giesbertz P, Meyer HE, Daniel H, Spanier B, Intestinal amino acid availability via PEPT-1 affects TORC1/2 signaling and the unfolded protein response. J Proteome Res, 13(8):3685-92(2014) [pubmed]

curator keyword: Biological

submitter keyword: Pept-1(lg601), N2(Bristol), Caenorhabditis elegans, ontogenesis, peptide transporter, target of rapamycin, shot-gun proteomics, amino acid homeostasis

Prof. Hannelore Daniel
contact affiliation	Molecular Nutrition, Technische Universität München, Gregor-Mendel-Str. 2, 85354 Freising, Germany
contact email	hannelore.daniel@tum.de
lab head
Gerhard Mayer
contact affiliation	Bioinformatics / Biostatistics
contact email	mayerg97@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000310

PRIDE project URI

• PRIDE
  1. PXD000310
     1. Label: PRIDE project
     2. Name: Quantitative proteome profiling of Caenorhabditis elegans pept-1(lg601) and N2(Bristol) during ontogenesis.