PXD000305

PXD000305 is an original dataset announced via ProteomeXchange.

Title	Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodelling in the Arabidopsis response to phosphate starvation
Description	In order to identify new regulators of the phosphate (Pi) starvation signaling pathway in plants, we analyzed variation in the abundance of nuclear-enriched nuclear proteins isolated from Arabidopsis roots that depends on Pi supply. We used 2-D Fluorescence Difference Gel Electrophoresis and MALDI-TOF/TOF techniques for nuclear proteome separation, visualization and relative protein abundance quantification and identification. Pi-controlled proteins identified in our analysis included components of the chromatin remodeling, DNA replication, and mRNA splicing machineries. In addition, by combining Pi starvation conditions with proteasome inhibitor treatments, we characterized the role of the ubiquitin- (Ub)-proteasome system (UPS), a major mechanism for targeted protein degradation in eukaryotes, in the control of the stability of Pi-responsive proteins. Among Pi-responsive proteins, the histone chaperone NAP1;2 was selected for further characterization, and was shown to display differential nucleo-cytoplasmic accumulation in response to Pi deprivation. We also found that mutants affecting three members of the NAP1 family accumulate lower Pi levels and display reduced expression of Pi starvation-inducible genes, reflecting a regulatory role for these chromatin-remodelling proteins in Pi homeostasis.
HostingRepository	PRIDE
AnnounceDate	2020-01-22
AnnouncementXML	Submission_2020-01-22_04:55:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000305
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vicente Rubio
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-27 05:05:31	ID requested
⏵ 1	2020-01-22 04:55:17	announced

Iglesias J, Trigueros M, Rojas-Triana M, Fern, á, ndez M, Albar JP, Bustos R, Paz-Ares J, Rubio V, Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation. J Proteomics, 94():1-22(2013) [pubmed]

submitter keyword: Phosphate starvation, Phosphate signaling, nuclear proteome, ubiquitin-proteasome system, NAP1;2, histone chaperone, DIGE, Arabidopsis

Vicente Rubio
contact affiliation	Centro Nacional de Biotecnología (CNB-CSIC), Darwin, 3, 28049 Madrid, Spain
contact email	vrubio@cnb.csic.es
lab head
Vicente Rubio
contact affiliation	Genetica Molecular de Plantas
contact email	vrubio@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000305
     1. Label: PRIDE project
     2. Name: Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodelling in the Arabidopsis response to phosphate starvation