PXD000302

PXD000302 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of urine and stone matrix of patients with kidney calculi
Description	Proteins have been extracted from kidney calculi with SDS buffer. Proteins were seperated by 1D-SDS-PAGE and stained with coomassie. Each gel lane was cut into 5 (or 6) pieces. Proteins were digested in gel with trypsin. Tryptic peptides were analysed by nanoLC-MS/MS on an ion trap instrument (6340 Ion Trap, Agilent) with CID. Peak lists were generated with Mascot Distiller, and database searching was performed with Mascot. The data from 5 (or 6) LC-MS/MS runs from one patient ware merged into one mgf file, which was searched against IPI (human) database.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_08:58:14.389.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000302
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andreas Schlosser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Glu->pyro-Glu; deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	1200 series LC/MSD SL

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-27 01:54:27	ID requested
1	2016-07-11 07:17:02	announced
⏵ 2	2024-10-07 08:58:15	announced	2024-10-07: Updated project metadata.

10.6019/PXD000302;

Boonla C, Tosukhowong P, Spittau B, Schlosser A, Pimratana C, Krieglstein K, Inflammatory and fibrotic proteins proteomically identified as key protein constituents in urine and stone matrix of patients with kidney calculi. Clin Chim Acta, 429():81-9(2014) [pubmed]

10.1016/j.cca.2013.11.036;

submitter keyword: inflammation,kidney stone, fibrosis, biomarker

Andreas Schlosser
contact affiliation	Rudolf Virchow Center for Experimental Biomedicine, Universität Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg
contact email	andreas.schlosser@virchow.uni-wuerzburg.de
lab head
Andreas Schlosser
contact affiliation	Mass Spectrometry and Proteomics
contact email	andreas.schlosser@virchow.uni-wuerzburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD000302

PRIDE project URI

• PRIDE
  1. PXD000302
     1. Label: PRIDE project
     2. Name: Proteomic analysis of urine and stone matrix of patients with kidney calculi