PXD000301

PXD000301 is an original dataset announced via ProteomeXchange.

Title	Calorie restriction-induced changes in the secretome of human adipocytes, comparison with resveratrol-induced secretome effects
Description	Obesity is characterized by excess energy storage which results in dysfunctional white adipose tissue (WAT) and in turn leads to metabolic diseases. Lifestyle changes especially calorie restriction (CR) reduces the risk for age and obesity-associated complications. The impact of CR on obesity has mainly been examined with human intervention studies, which indicated alterations in circulating adipokines. However, a detailed understanding of CR-induced adipocyte secretome changes remains elusive. Therefore, we investigated the effect of CR on the secretion profile of mature human adipocytes by using proteomics technology with bioinformatic analysis. We demonstrated CR-mediated adipocyte triglyceride reduction, which resulted in a positive effect on adipokine secretion indicating an improved inflammatory phenotype and alleviation of obesity-associated metabolic dysfunction including insulin resistance and glucose intolerance. Furthermore, 6 novel adipocyte-secreted proteins were identified which were regulated by CR. Since resveratrol (RSV) mimics CR we compared results from this study with data from our previous RSV study on the adipocyte secretome. We observed that both treatment strategies lead to a less inflammatory phenotype and an altered adipokine profile indicating improvement of metabolic complications even though the CR and RSV adipocyte secretomes differed from each other.
HostingRepository	PRIDE
AnnounceDate	2016-06-07
AnnouncementXML	Submission_2016-06-07_04:06:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000301
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean-Paul Noben
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-26 01:46:04	ID requested
1	2016-05-25 11:48:42	announced
⏵ 2	2016-06-07 04:06:52	announced	Updated project metadata.

Renes J, Rosenow A, Roumans N, Noben JP, Mariman EC, Calorie restriction-induced changes in the secretome of human adipocytes, comparison with resveratrol-induced secretome effects. Biochim Biophys Acta, 1844(9):1511-22(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: human adipocytes, calorie restriction, adipokines, metabolic syndrome, 2-DE LC-MS/MS

Prof. Dr. E. Mariman
contact affiliation	Dept. of Human Biology, FHML, Maastricht University, P.O. Box 616, 6200 MD, Maastricht, The Netherlands
contact email	e.mariman@maastrichtuniversity.nl
lab head
Jean-Paul Noben
contact affiliation	FBI
contact email	jeanpaul.noben@uhasselt.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000301
     1. Label: PRIDE project
     2. Name: Calorie restriction-induced changes in the secretome of human adipocytes, comparison with resveratrol-induced secretome effects