PXD000299

PXD000299 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of fluids from the genital tract of ewes during the estrous cycle
Description	Fluids samples of oviduct, uterus and cervical mucus were collected in sheep D0 (J0) and D10 (J10) in spontaneous (N) and synchronized (I) by flushing with PBS 1X. Proteins for each fluid were included in-gel (3-4 bands) followed by Coomassie Blue staining. After reduction and alkylation, proteins were in-gel digested with trypsin and peptide mixtures were analyzed by nanoLC-MS/MS using LTQ velos Orbitrap mass spectrometer. Raw data files were converted to MGF with Proteome Discoverer software (version 1.2; Thermo Fischer Scientific, San Jose, USA). Precursor mass range of 350-5000 Da and signal to noise ratio of 1.5 were the criteria used for generation of peak lists. In order to identify the proteins, the peptide and fragment masses obtained were matched automatically against the mammalia section of a locally maintained copy of nr NCBI (01/01/2013). MS/MS ion searches were performed using MASCOT Daemon and search engine (version 2.3; Matrix Science, London, UK). The parameters used for database searches include trypsin as a protease with allowed two missed cleavage, carbamidomethylcysteine (+57 Da), oxidation of methionine (+16) and N-terminal protein acetylation (+42) as variable modifications. The tolerance of the ions was set to 5 ppm for parent and 0.8 Da for fragment ion matches
HostingRepository	PRIDE
AnnounceDate	2015-11-05
AnnouncementXML	Submission_2015-11-05_07:38:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	G HARICHAUX
SpeciesList	scientific name: Ovis aries (Sheep); NCBI TaxID: 9940;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-25 02:10:52	ID requested
⏵ 1	2015-11-05 07:38:49	announced

Soleilhavoup C, Riou C, Tsikis G, Labas V, Harichaux G, Kohnke P, Reynaud K, de Graaf SP, Gerard N, Druart X, Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics, 15(1):93-108(2016) [pubmed]

submitter keyword: LC-MS/MS, cervical mucus, uterus, oviduct, fertility, reproduction

G HARICHAUX
contact affiliation	INRA-Nouzilly-PRC
contact email	gregoire.harichaux@tours.inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD000299

PRIDE project URI

• PRIDE
  1. PXD000299
     1. Label: PRIDE project
     2. Name: Proteomic analysis of fluids from the genital tract of ewes during the estrous cycle