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PXD000289

PXD000289 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSulfolobus acidocaldarius phosphoproteome
DescriptionShotgun phosphoproteome of Sulfolobus acidocaldarius using PAciFIC technique. Briefly, proteins were denatured, alkylated, trypsine digestion, SCX separation Fractionated peptides were analysed on Bruker HCTultra. Data processing and bioinformatics: data from mass spectrometry were then extracted to mgf format using Bruker Data Analysis V4.0 with a MRM script, these were then were searched against the Sulfolobus acidocaldarius database (containing 2223 proteins) downloaded from NCBI in March 2010 using Phenyx V 2.6 (Genebio, Geneva). The searches were performed using parameters as follows: carbamidomethylation of cysteine (fixed modification), oxidation of methionine (variation), and phosphorylation of serine, tyrosine, threonine (variation), trypsin with 2 missed cleavages. Furthermore, other parameters such as parent, MS/MS, tolerances were set at 2.0 and 0.8 Da, respectively, whilst minimum peptide length, z-score, p-value and AC score were set at 5, 5.5, 10-5, and 5.5 respectively.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_03:47:49.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTrong Khoa Pham
SpeciesList scientific name: Sulfolobus acidocaldarius; NCBI TaxID: 2285;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
InstrumentBruker Daltonics HCT Series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-06-17 06:39:40ID requested
12014-04-15 04:11:34announced
22014-07-24 03:47:50announcedUpdated project metadata.
Publication List
Reimann J, Esser D, Orell A, Amman F, Pham TK, Noirel J, Lind, å, s AC, Bernander R, Wright PC, Siebers B, Albers SV, Archaeal signal transduction: impact of protein phosphatase deletions on cell size, motility, and energy metabolism in Sulfolobus acidocaldarius. Mol Cell Proteomics, 12(12):3908-23(2013) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Saci, Phosphoproteome, mutant strains
Contact List
Trong Khoa Pham
contact affiliationDepartment of Chemical and Biological Engineering
contact emailkhoa.pham@sheffield.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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