PXD000289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sulfolobus acidocaldarius phosphoproteome |
Description | Shotgun phosphoproteome of Sulfolobus acidocaldarius using PAciFIC technique. Briefly, proteins were denatured, alkylated, trypsine digestion, SCX separation Fractionated peptides were analysed on Bruker HCTultra. Data processing and bioinformatics: data from mass spectrometry were then extracted to mgf format using Bruker Data Analysis V4.0 with a MRM script, these were then were searched against the Sulfolobus acidocaldarius database (containing 2223 proteins) downloaded from NCBI in March 2010 using Phenyx V 2.6 (Genebio, Geneva). The searches were performed using parameters as follows: carbamidomethylation of cysteine (fixed modification), oxidation of methionine (variation), and phosphorylation of serine, tyrosine, threonine (variation), trypsin with 2 missed cleavages. Furthermore, other parameters such as parent, MS/MS, tolerances were set at 2.0 and 0.8 Da, respectively, whilst minimum peptide length, z-score, p-value and AC score were set at 5, 5.5, 10-5, and 5.5 respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_03:47:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Trong Khoa Pham |
SpeciesList | scientific name: Sulfolobus acidocaldarius; NCBI TaxID: 2285; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue |
Instrument | Bruker Daltonics HCT Series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-06-17 06:39:40 | ID requested | |
1 | 2014-04-15 04:11:34 | announced | |
⏵ 2 | 2014-07-24 03:47:50 | announced | Updated project metadata. |
Publication List
Reimann J, Esser D, Orell A, Amman F, Pham TK, Noirel J, Lind, å, s AC, Bernander R, Wright PC, Siebers B, Albers SV, Archaeal signal transduction: impact of protein phosphatase deletions on cell size, motility, and energy metabolism in Sulfolobus acidocaldarius. Mol Cell Proteomics, 12(12):3908-23(2013) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saci, Phosphoproteome, mutant strains |
Contact List
Trong Khoa Pham |
contact affiliation | Department of Chemical and Biological Engineering |
contact email | khoa.pham@sheffield.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000289
- Label: PRIDE project
- Name: Sulfolobus acidocaldarius phosphoproteome