PXD000265 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Oryza sativa egg, sperm, callus, pollen and seedling proteome |
Description | In angiosperms, female gamete differentiation, fertilization, and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries. Despite their importance in plant reproduction and development, how the egg cell is specialized, fuses with the sperm cell, and converts into an active zygote for early embryogenesis remains unclear. This lack of knowledge is partly attributable to the difficulty of direct analyses of gametes in angiosperms. In this study, proteins from egg and sperm cells obtained from Oryza sativa flowers were separated by one-dimensional polyacrylamide gel electrophoresis and globally identified by highly sensitive liquid chromatography coupled with tandem mass spectroscopy. Proteome analyses were also conducted for seedlings, callus, and pollen grains to compare their protein expression profiles to those of gametes. Database was searched using Mascot software (ver.2.2.1, Matrix Science, MA, USA) with the following parameters. The fixed modification was propionasmide (Cys) and variable modification parameters were pyro-Glu, acetylation (protein N-terminus), and oxidation (Met). The maximum missed cleavage was set at 3 with a peptide mass tolerance of +/– 15 ppm. Peptide charges from +2 to +4 states and MS/MS tolerances of +/– 0.8 Da were allowed. |
HostingRepository | PRIDE |
AnnounceDate | 2013-08-14 |
AnnouncementXML | Submission_2013-08-14_05:27:55.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000265 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Masato Taoka |
SpeciesList | scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; |
ModificationList | monohydroxylated residue; acetylated residue; neutral loss: 63.998285; S-carboxamidoethyl-L-cysteine; deaminated residue |
Instrument | radial ejection linear ion trap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-05-23 02:35:11 | ID requested | |
1 | 2013-08-02 04:42:27 | announced | |
1 | 2014-07-28 00:38:42 | announced | |
⏵ 2 | 2013-08-14 05:27:55 | announced | Add reference |
Publication List
Abiko M, Furuta K, Yamauchi Y, Fujita C, Taoka M, Isobe T, Okamoto T, Identification of proteins enriched in rice egg or sperm cells by single-cell proteomics. PLoS One, 8(7):e69578(2013) [pubmed] |
Keyword List
submitter keyword: O. sativa, egg, sperm, callus, pollen, seedling LC-MS/MS |
Contact List
Masato Taoka |
contact affiliation | Graduate School of Science and Engineering, Tokyo Metropolitan University |
contact email | mango@tmu.ac.jp |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/08/PXD000265 |
Repository Record List
[ + ]
[ - ]
- PRIDE
- 29572
- InstrumentRef: INSTRUMENT_1
- Label: Gel25
- ModificationList: S-carboxamidoethyl-L-cysteine, monohydroxylated residue,
- Name: 110603okamoto25.mgf-ine.xls
- PublicationRef: PMID23936051
- 29560
- InstrumentRef: INSTRUMENT_1
- Label: Gel1-2
- ModificationList: monohydroxylated residue, acetylated residue, neutral loss,
- Name: 110603okamoto12.mgf-ine.xls
- PublicationRef: PMID23936051
- 29608
- InstrumentRef: INSTRUMENT_1
- Label: Gel5-6
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, acetylated residue, neutral loss,
- Name: 120118P03.mgf-121336.xls
- PublicationRef: PMID23936051
- 29597
- InstrumentRef: INSTRUMENT_1
- Label: Gel5-6
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue,
- Name: 120306okamoto-M03.mgf-104615.xls
- PublicationRef: PMID23936051
- 29573
- InstrumentRef: INSTRUMENT_1
- Label: Gel1-2
- ModificationList: deaminated residue, monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120110C-01.mgf-104144.xls
- PublicationRef: PMID23936051
- 29591
- InstrumentRef: INSTRUMENT_1
- Label: Gel15-16
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120112S08.mgf-102925.xls
- PublicationRef: PMID23936051
- 29609
- InstrumentRef: INSTRUMENT_1
- Label: Gel7-8
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 120118P04.mgf-121511.xls
- PublicationRef: PMID23936051
- 29562
- InstrumentRef: INSTRUMENT_1
- Label: Gel5-6
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine,
- Name: 110603okamoto56.mgf-ine.xls
- PublicationRef: PMID23936051
- 29596
- InstrumentRef: INSTRUMENT_1
- Label: Gel3-4
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120306okamoto-M02.mgf-104535.xls
- PublicationRef: PMID23936051
- 29604
- InstrumentRef: INSTRUMENT_1
- Label: Gel19-20
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 120306okamoto-M10.mgf-105434.xls
- PublicationRef: PMID23936051
- 29574
- InstrumentRef: INSTRUMENT_1
- Label: Gel3-4
- ModificationList: deaminated residue, monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120110C-02.mgf-104412.xls
- PublicationRef: PMID23936051
- 29603
- InstrumentRef: INSTRUMENT_1
- Label: Gel17-18
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue,
- Name: 120306okamoto-M09.mgf-105329.xls
- PublicationRef: PMID23936051
- 29583
- InstrumentRef: INSTRUMENT_1
- Label: Gel21-22
- ModificationList: deaminated residue, monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120110C-11.mgf-105808.xls
- PublicationRef: PMID23936051
- 29605
- InstrumentRef: INSTRUMENT_1
- Label: Gel21-22
- ModificationList: acetylated residue, monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120306okamoto-M11.mgf-105517.xls
- PublicationRef: PMID23936051
- 29563
- InstrumentRef: INSTRUMENT_1
- Label: Gel7-8
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, neutral loss,
- Name: 110603okamoto78.mgf-ine.xls
- PublicationRef: PMID23936051
- 29592
- InstrumentRef: INSTRUMENT_1
- Label: Gel17-18
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120112S09.mgf-102949.xls
- PublicationRef: PMID23936051
- 29569
- InstrumentRef: INSTRUMENT_1
- Label: Gel19-20
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 110603okamoto1920.mgf-ine.xls
- PublicationRef: PMID23936051
- 29595
- InstrumentRef: INSTRUMENT_1
- Label: Gel1-2
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, neutral loss, acetylated residue, deaminated residue,
- Name: 120306okamoto-M01.mgf-104512.xls
- PublicationRef: PMID23936051
- 29578
- InstrumentRef: INSTRUMENT_1
- Label: Gel11-12
- ModificationList: deaminated residue, monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120110C-06.mgf-105209.xls
- PublicationRef: PMID23936051
- 29564
- InstrumentRef: INSTRUMENT_1
- Label: Gel9-10
- ModificationList: monohydroxylated residue, deaminated residue, acetylated residue, S-carboxamidoethyl-L-cysteine,
- Name: 110603okamoto910.mgf-ine.xls
- PublicationRef: PMID23936051
- 29607
- InstrumentRef: INSTRUMENT_1
- Label: Gel3-4
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120118P02.mgf-121217.xls
- PublicationRef: PMID23936051
- 29568
- InstrumentRef: INSTRUMENT_1
- Label: Gel17-18
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 110603okamoto1718.mgf-ine.xls
- PublicationRef: PMID23936051
- 29589
- InstrumentRef: INSTRUMENT_1
- Label: Gel11-12
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss, acetylated residue,
- Name: 120112S06.mgf-102817.xls
- PublicationRef: PMID23936051
- 29612
- InstrumentRef: INSTRUMENT_1
- Label: Gel13-14
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120118P07.mgf-121819.xls
- PublicationRef: PMID23936051
- 29582
- InstrumentRef: INSTRUMENT_1
- Label: Gel19-20
- ModificationList: S-carboxamidoethyl-L-cysteine, deaminated residue, monohydroxylated residue, acetylated residue, neutral loss,
- Name: 120110C-10.mgf-105717.xls
- PublicationRef: PMID23936051
- 29611
- InstrumentRef: INSTRUMENT_1
- Label: Gel11-12
- ModificationList: monohydroxylated residue, deaminated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120118P06.mgf-121727.xls
- PublicationRef: PMID23936051
- 29593
- InstrumentRef: INSTRUMENT_1
- Label: Gel19-20
- ModificationList: monohydroxylated residue, deaminated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120112S10.mgf-103009.xls
- PublicationRef: PMID23936051
- 29590
- InstrumentRef: INSTRUMENT_1
- Label: Gel13-14
- ModificationList: monohydroxylated residue, deaminated residue, acetylated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120112S07.mgf-102859.xls
- PublicationRef: PMID23936051
- 29614
- InstrumentRef: INSTRUMENT_1
- Label: Gel17-18
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, neutral loss,
- Name: 120118P09.mgf-121910.xls
- PublicationRef: PMID23936051
- 29594
- InstrumentRef: INSTRUMENT_1
- Label: Gel21-22
- ModificationList: acetylated residue, monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120112S11.mgf-103029.xls
- PublicationRef: PMID23936051
- 29580
- InstrumentRef: INSTRUMENT_1
- Label: Gel15-16
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120110C-08.mgf-105501.xls
- PublicationRef: PMID23936051
- 29567
- InstrumentRef: INSTRUMENT_1
- Label: Gel15-16
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, acetylated residue,
- Name: 110603okamoto1516.mgf-ine.xls
- PublicationRef: PMID23936051
- 29616
- InstrumentRef: INSTRUMENT_1
- Label: Gel21-22
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120118P11.mgf-122012.xls
- PublicationRef: PMID23936051
- 29579
- InstrumentRef: INSTRUMENT_1
- Label: Gel13-14
- ModificationList: deaminated residue, acetylated residue, monohydroxylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120110C-07.mgf-105352.xls
- PublicationRef: PMID23936051
- 29601
- InstrumentRef: INSTRUMENT_1
- Label: Gel13-14
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 120306okamoto-M07.mgf-105140.xls
- PublicationRef: PMID23936051
- 29581
- InstrumentRef: INSTRUMENT_1
- Label: Gel17-18
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120110C-09.mgf-105605.xls
- PublicationRef: PMID23936051
- 29565
- InstrumentRef: INSTRUMENT_1
- Label: Gel11-12
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue,
- Name: 110603okamoto1112mgf-.ine.xls
- PublicationRef: PMID23936051
- 29599
- InstrumentRef: INSTRUMENT_1
- Label: Gel9-10
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue,
- Name: 120306okamoto-M05.mgf-104849.xls
- PublicationRef: PMID23936051
- 29588
- InstrumentRef: INSTRUMENT_1
- Label: Gel9-10
- ModificationList: acetylated residue, S-carboxamidoethyl-L-cysteine, monohydroxylated residue, deaminated residue,
- Name: 120112S05.mgf-102737.xls
- PublicationRef: PMID23936051
- 29577
- InstrumentRef: INSTRUMENT_1
- Label: Gel9-10
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120110C-05.mgf-105018.xls
- PublicationRef: PMID23936051
- 29615
- InstrumentRef: INSTRUMENT_1
- Label: Gel19-20
- ModificationList: monohydroxylated residue, deaminated residue, neutral loss, acetylated residue, S-carboxamidoethyl-L-cysteine,
- Name: 120118P10.mgf-121938.xls
- PublicationRef: PMID23936051
- 29584
- InstrumentRef: INSTRUMENT_1
- Label: Gel1-2
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue,
- Name: 120112S01.mgf-102446.xls
- PublicationRef: PMID23936051
- 29570
- InstrumentRef: INSTRUMENT_1
- Label: Gel21-22
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, deaminated residue, neutral loss,
- Name: 110603okamoto2122.mgf-ine.xls
- PublicationRef: PMID23936051
- 29585
- InstrumentRef: INSTRUMENT_1
- Label: Gel3-4
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue,
- Name: 120112S02.mgf-102523.xls
- PublicationRef: PMID23936051
- 29587
- InstrumentRef: INSTRUMENT_1
- Label: Gel7-8
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, acetylated residue,
- Name: 120112S04.mgf-102647.xls
- PublicationRef: PMID23936051
- 29571
- InstrumentRef: INSTRUMENT_1
- Label: Gel23-24
- ModificationList: deaminated residue, acetylated residue, monohydroxylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 110603okamoto2324.mgf-ine.xls
- PublicationRef: PMID23936051
- 29602
- InstrumentRef: INSTRUMENT_1
- Label: Gel15-16
- ModificationList: monohydroxylated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue,
- Name: 120306okamoto-M08.mgf-105229.xls
- PublicationRef: PMID23936051
- 29586
- InstrumentRef: INSTRUMENT_1
- Label: Gel5-6
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, neutral loss, acetylated residue,
- Name: 120112S03.mgf-102546.xls
- PublicationRef: PMID23936051
- 29606
- InstrumentRef: INSTRUMENT_1
- Label: Gel1-2
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, acetylated residue,
- Name: 120118P01.mgf-121123.xls
- PublicationRef: PMID23936051
- 29598
- InstrumentRef: INSTRUMENT_1
- Label: Gel7-8
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue, acetylated residue,
- Name: 120306okamoto-M04.mgf-104728.xls
- PublicationRef: PMID23936051
- 29600
- InstrumentRef: INSTRUMENT_1
- Label: Gel11-12
- ModificationList: monohydroxylated residue, neutral loss, acetylated residue, S-carboxamidoethyl-L-cysteine, deaminated residue,
- Name: 120306okamoto-M06.mgf-105006.xls
- PublicationRef: PMID23936051
- 29575
- InstrumentRef: INSTRUMENT_1
- Label: Gel5-6
- ModificationList: monohydroxylated residue, deaminated residue, acetylated residue, S-carboxamidoethyl-L-cysteine, neutral loss,
- Name: 120110C-03.mgf-104633.xls
- PublicationRef: PMID23936051
- 29561
- InstrumentRef: INSTRUMENT_1
- Label: Gel3-4
- ModificationList: monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue,
- Name: 110603okamoto34.mgf-ine.xls
- PublicationRef: PMID23936051
- 29613
- InstrumentRef: INSTRUMENT_1
- Label: Gel15-16
- ModificationList: monohydroxylated residue, deaminated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120118P08.mgf-121848.xls
- PublicationRef: PMID23936051
- 29576
- InstrumentRef: INSTRUMENT_1
- Label: Gel7-8
- ModificationList: deaminated residue, monohydroxylated residue, S-carboxamidoethyl-L-cysteine, acetylated residue, neutral loss,
- Name: 120110C-04.mgf-104851.xls
- PublicationRef: PMID23936051
- 29610
- InstrumentRef: INSTRUMENT_1
- Label: Gel9-10
- ModificationList: S-carboxamidoethyl-L-cysteine, acetylated residue, monohydroxylated residue, deaminated residue, neutral loss,
- Name: 120118P05.mgf-121634.xls
- PublicationRef: PMID23936051
- 29566
- InstrumentRef: INSTRUMENT_1
- Label: Gel13-14
- ModificationList: monohydroxylated residue, acetylated residue, deaminated residue, neutral loss, S-carboxamidoethyl-L-cysteine,
- Name: 110603okamoto1314.mgf-ine.xls
- PublicationRef: PMID23936051