PXD000265

PXD000265 is an original dataset announced via ProteomeXchange.

Title	Oryza sativa egg, sperm, callus, pollen and seedling proteome
Description	In angiosperms, female gamete differentiation, fertilization, and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries. Despite their importance in plant reproduction and development, how the egg cell is specialized, fuses with the sperm cell, and converts into an active zygote for early embryogenesis remains unclear. This lack of knowledge is partly attributable to the difficulty of direct analyses of gametes in angiosperms. In this study, proteins from egg and sperm cells obtained from Oryza sativa flowers were separated by one-dimensional polyacrylamide gel electrophoresis and globally identified by highly sensitive liquid chromatography coupled with tandem mass spectroscopy. Proteome analyses were also conducted for seedlings, callus, and pollen grains to compare their protein expression profiles to those of gametes. Database was searched using Mascot software (ver.2.2.1, Matrix Science, MA, USA) with the following parameters. The fixed modification was propionasmide (Cys) and variable modification parameters were pyro-Glu, acetylation (protein N-terminus), and oxidation (Met). The maximum missed cleavage was set at 3 with a peptide mass tolerance of +/– 15 ppm. Peptide charges from +2 to +4 states and MS/MS tolerances of +/– 0.8 Da were allowed.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_08:56:57.508.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000265
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Masato Taoka
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	S-carboxamidoethyl-L-cysteine; deaminated residue; acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2013-05-23 02:35:11	ID requested
1	2013-08-02 04:42:27	announced
1	2014-07-28 00:38:42	announced
2	2013-08-14 05:27:55	announced	Add reference
⏵ 3	2024-10-07 08:56:58	announced	2024-10-07: Updated project metadata.

10.6019/PXD000265;

Abiko M, Furuta K, Yamauchi Y, Fujita C, Taoka M, Isobe T, Okamoto T, Identification of proteins enriched in rice egg or sperm cells by single-cell proteomics. PLoS One, 8(7):e69578(2013) [pubmed]

10.1371/journal.pone.0069578;

submitter keyword: seedling LC-MS/MS, egg, sperm,O. sativa, callus, pollen

Masato Taoka
contact affiliation	Graduate School of Science and Engineering
contact email	mango@tmu.ac.jp
lab head
Masato Taoka
contact affiliation	Graduate School of Science and Engineering
contact email	mango@tmu.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000265
     1. Label: PRIDE project
     2. Name: Oryza sativa egg, sperm, callus, pollen and seedling proteome