PXD000263

PXD000263 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of the MO3.13 cell line from human oligodendrocytes
Description	MO3.13 is an immortal human-human hybrid cell line that express phenotypic characteristics of primary oligodendrocytes. It was created by fusing a 6-thioguanine-resistant mutant of the human rhabdomyosarcoma RD (cancer of skeletal muscle) with adult human oligodendrocytes by a lectin-enhanced polyethylene glycol procedure. In contrast to the tumor parent, MO3.13 expressed surface immunoreactivity for galactosyl cerebroside(GS) and intracellular immunoreactivity for myelin basic protein (MBP), proteolipid protein (PLP), and glial fibrillary acidic protein (GFAP). MO3.13 also exhibit the markers of immature oligodendrocytes GalC (galactosylceramidase) and CNPase. Upon differentiation, the MO3.13 cells have been also shown to express the MBP and MOG markers. Data analysis: Each MS raw file was processed using ProteoWizard for the generation of a MGF file. These were processed in SearchGUI, which runs the search engines Open Mass Spectrometry Search Algorithm (OMSSA) and X!Tandem against the UniProt human protein database (release 2013_08, 20,266 sequences).  Search parameters were: peptide and fragment ion mass accuracy 10 ppm and 0.5 Da, respectively; protein and peptide FDRs 1%; two miss cleavages; trypsin as enzyme; fixed modifications: cysteine carbamidomethylation; variable modifications: methionine oxidation. In order to generate one single proteome dataset of MO3.13, resulting data was processed in PeptideShaker.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_01:53:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000263
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniel Martins-de-Souza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized amino-terminal residue: 57.021464; monohydroxylated residue: 15.994915
Instrument	LTQ Orbitrap; instrument model: LTQ-Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-05-22 03:22:11	ID requested
1	2013-10-09 08:08:25	announced
1	2013-11-07 08:51:19	announced
2	2014-07-28 00:38:04	announced	Updated project metadata.
⏵ 3	2014-08-08 01:53:12	announced	Updated project metadata.

Iwata K, Caf, é, -Mendes CC, Schmitt A, Steiner J, Manabe T, Matsuzaki H, Falkai P, Turck CW, Martins-de-Souza D, The human oligodendrocyte proteome. Proteomics, 13(23-24):3548-53(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: Human

oligodendrocytes

proteome

C-HPP

Daniel Martins-de-Souza
contact affiliation	Chemical Engineering and Biotechnology
contact email	danms90@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000263
     1. Label: PRIDE project
     2. Name: Proteome analysis of the MO3.13 cell line from human oligodendrocytes