update LabHead growth: Exponential phase cultures of Mycobacterium bovis BCG grown in Middlebrook 7H9 supplemented with 0.3% glycerol, 0.085% NaCl, 0.05% tyloxapol treatment: Standing culture (shaking was stopped for 6 days) extraction: Lysis buffer containing 8M Urea and 0.1% RapiGest. Bead-beating to break up the cells (3 x 10 min). informatics: Manual refinement using Skyline massModifications: static: C+57.021464, variable: K+8.014199, variable: R+10.008269