update LabHead Exponential and stationary phase cultures of Mycobacterium tuberculosis grown in Middlebrook 7H9 supplemented with 0.3% glycerol, 0.085% NaCl, 0.05% tyloxapol.Lysis buffer containing 8M Urea and 0.1% RapiGest. Bead-beating to break up the cells (3 x 10 min).