PXD000241

PXD000241 is an original dataset announced via ProteomeXchange.

Title	Serine Hydrolases from E. Coli
Description	Serine hydrolases from E. Coli cells were labelled with fluorophosphonate probe (ABP-probe): Intact cells labelling by adding probe to media: GPM77700012901 and GPM00300015270 Cell lysate labelling: GPM77700012904 and GPM77700012905 Blank sample (no probe addition): GPM77700012906 and GPM77700012909 2D-LC-MS experiment was done with C18-chromatography at pH10 for first dimension (0.66% AcN/min gradient). Fractions (1 min each) from the first dimension were collected and concatenated: 1,2,3 and 4: 00-EColi2DFASP 45,46 and 47: 99-EColi2DFASP 5 and 25: 01-EColi2DFASP 6 and 26: 02-EColi2DFASP 7 and 27: 03-EColi2DFASP 24 and 44: 20-EColi2DFASP Concatenated fractions were dryed, redissolved in H2O-FA-AcN 97.9:0.1:2% and analysed with LC-MS using C18-chromatography at pH=2 as a second-dimension separation method. Combined datasearch results for 2D-LC-MS of E. Coli Dh5a could be seen on local laboratory server: http://140.193.59.4/tandem/thegpm_tandem.html with lookup model ID: GPM22200000195
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:47:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dmitry Shamshurin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2013-05-05 11:47:32	ID requested
1	2014-05-20 03:29:14	announced
⏵ 2	2014-07-24 03:47:18	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Serine Hydrolases, E. Coli, fluorophosphonate probe, Activity-based protein profiling

Dmitry Shamshurin
contact affiliation	Internal Medicine
contact email	dimsham@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/05/PXD000241

PRIDE project URI

• PRIDE
  1. PXD000241
     1. Label: PRIDE project
     2. Name: Serine Hydrolases from E. Coli