PXD000235

PXD000235 is an original dataset announced via ProteomeXchange.

Title	bHLH Transcription Factors that Facilitate K+ Uptake During Stomatal Opening are Repressed by Abscisic Acid through Phosphorylation
Description	Stomata open in response to light and close following exposure to abscisic acid (ABA). They regulate gas exchange between plants and atmosphere, allowing plants to adapt to changing environmental conditions. ABA binding to receptors initiates a signaling cascade that involves protein phosphorylation. Here we show that ABA induced phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-RESPONSIVE KINASE SUBSTRATES; AKS1, AKS2, AKS3), in Arabidopsis guard cells, and that they facilitated stomatal opening through the transcription of genes encoding inwardly-rectifying K+ channels. aks1aks2-1 double mutant plants showed decreases in light-induced stomatal opening, K+ accumulation in response to light, activity of inwardly-rectifying K+ channels, and transcription of genes encoding major inwardly-rectifying K+ channels without affecting ABA-mediated stomatal closure. Overexpression of POTASSIUM CHANNEL IN ARABIDOPSIS THALIANA 1 (KAT1), which encodes a major inwardly-rectifying K+ channel in guard cells, rescued the phenotype of aks1aks2-1 plants. AKS1 bound directly to the promoter of KAT1, an interaction that was attenuated after ABA-induced phosphorylation. The ABA agonist pyrabactin induced phosphorylation of AKSs. Our results demonstrate that the AKS family of bHLH transcription factors facilitates stomatal opening through transcription of genes encoding inwardly-rectifying K+ channels, and that ABA suppresses the activity of inwardly-rectifying K+ channel activity by triggering the phosphorylation of these transcription factors. Microarray data have been deposited in the Gene Expression Omnibus with accession number: GSE46574.
HostingRepository	PRIDE
AnnounceDate	2013-06-28
AnnouncementXML	Submission_2013-06-28_05:47:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000235
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohei Takahashi
SpeciesList	scientific name: Arabidopsis thaliana (thale cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	linear ion trap

Revision	Datetime	Status	ChangeLog Entry
0	2013-05-01 02:29:46	ID requested
1	2013-06-28 04:30:20	announced
1	2014-07-28 00:36:22	announced
⏵ 2	2013-06-28 05:47:12	announced	Add reference

Takahashi Y, Ebisu Y, Kinoshita T, Doi M, Okuma E, Murata Y, Shimazaki K, uptake during stomatal opening are repressed by abscisic acid through phosphorylation. Sci Signal, 6(280):ra48(2013) [pubmed]

submitter keyword: Arabidopsis, stomatal guard cells, LTQ

Yohei Takahashi
contact affiliation	Kyushu university
contact email	takahashircb@gmail.com

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• PRIDE
  1. 29513
     1. InstrumentRef: INSTRUMENT_1
     2. Label: 53kd band
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Identification of AKSs
     5. PublicationRef: PMID23779086
  2. 29512
     1. InstrumentRef: INSTRUMENT_1
     2. Label: 43kd band
     3. ModificationList: iodoacetamide derivatized residue,
     4. Name: Identification of AKSs
     5. PublicationRef: PMID23779086