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PXD000217

PXD000217 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics data classify haematological cancer cell lines according to tumour type and sensitivity to kinase inhibitors
DescriptionAcute myeloid leukemia, lymphoma and multiple myeloma cell lines were grown for 24h in RPMI medium and cells were harvested by centrifugation. Samples were digested with trypsin and subjected to phosphoenrichment using IMAC. Phosphopeptides were run in a LTQ-Orbitrap-XL. Peaks lists were generated with Mascot Distiller (version 2.3) in MGF format and Database searches were with Mascot Server (version 2.3) against the SwissProt database restricted to human sequences (release December 2011) and trypsin cleavage. Restrictions were 7ppm for parent ions and 600 mmu for fragment masses. Allowed modifications were phosphorylation of Ser/Thr/Tyr, pyro-Glu (N-term) and methionine oxidation and one miss-cleavage allowed. Quantification was by label-free using peak heights of extracted ion chromatograms (XICs) constructed with narrow mass windows (7ppm) and time windows (1.5 minutes). Pescal, a computer program written in house, was used to automate the generation of XICs and to calculate peak heights.
HostingRepositoryPRIDE
AnnounceDate2013-05-01
AnnouncementXMLSubmission_2013-05-01_04:46:38.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000217
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPedro Casado-Izquierdo
SpeciesList scientific name: Homo sapiens (human); NCBI TaxID: 9606;
ModificationListdeaminated residue; monohydroxylated residue; phosphorylated residue; neutral loss: 97.976896; iodoacetamide derivatized residue; neutral loss: 63.998285
Instrumentradial ejection linear ion trap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-04-19 02:20:03ID requested
12013-04-26 06:58:28announced
12014-07-28 00:35:06announced
22013-05-01 04:46:38announcedAdd reference
Publication List
Casado P, Alcolea MP, Iorio F, Rodr, í, guez-Prados JC, Vanhaesebroeck B, Saez-Rodriguez J, Joel S, Cutillas PR, Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol, 14(4):R37(2013) [pubmed]
Keyword List
submitter keyword: Phosphoproteomics, IMAC, Kinase inhibitor, Drug sensitivity, Hierarchical Clustering Analysis, Blood Malignancies, Acute Myeloid Leukemia
Contact List
pedro cutillas
contact attributepedro.cutillas@csc.mrc.ac.uk
contact affiliationMRC Imperial Colledge
contact emailpedro.cutillas@csc.mrc.ac.uk
Pedro Casado-Izquierdo
contact affiliationCell Signalling
contact emailp.m.casado-izquierdo@qmul.ac.uk
Full Dataset Link List
Dataset FTP location
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Repository Record List
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