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PXD000205

PXD000205 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteoRed Multicentric Experiment for Proteomics Quality Control
DescriptionProteomic technologies based on mass spectrometry (MS) have greatly evolved in the past years, and nowadays it is possible to routinely identify thousands of peptides from complex biological samples in a single LC-MS/MS experiment. Despite the advancements in proteomic technologies, the scientific community still faces important challenges in terms of depth and reproducibility of proteomics analyses. Here, we present a multicenter study designed to evaluate long-term performance of LC-MS/MS platforms within the Spanish Proteomics Facilities Network (ProteoRed-ISCIII). The study was performed under well-established standard operating procedures, and demonstrated that it is possible to attain high qualitative and quantitative reproducibility over time. Our study highlights the importance of deploying platform quality assessment metrics in multi-laboratory studies in early LC-MS/MS system troubleshooting.
HostingRepositoryPRIDE
AnnounceDate2015-07-20
AnnouncementXMLSubmission_2015-07-20_14:42:01.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSalvador Martinez-Bartolome
SpeciesList scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: 1247190;
ModificationListOxidation; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylix acid (Pro); Carbamidomethyl
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-04-08 03:41:30ID requested
12015-07-20 14:42:02announced
Publication List
Campos A, D, í, az R, Mart, í, nez-Bartolom, é S, Sierra J, Gallardo O, Sabid, ó E, L, ó, pez-Lucendo M, Ignacio Casal J, Pasquarello C, Scherl A, Chiva C, Borras E, Odena A, Elortza F, Azkargorta M, Ibarrola N, Canals F, Albar JP, Oliveira E, Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers. J Proteomics, 127(Pt B):264-74(2015) [pubmed]
Keyword List
submitter keyword: Multicentric, QC, Reproducibility, Orbitrap
Contact List
Eliandre de Oliveira
contact affiliationProteoRed-ISCIII, Proteomics Platform, Barcelona Science Park, Barcelona, Spain.
contact emaileoliveira@pcb.ub.es
lab head
Salvador Martinez-Bartolome
contact affiliationProteoRed-ISCIII, Proteomics Facility, Centro Nacional de Biotecnología - CSIC, Madrid, Spain.
contact emailsmartinez@proteored.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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