PXD000205
PXD000205 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | ProteoRed Multicentric Experiment for Proteomics Quality Control |
Description | Proteomic technologies based on mass spectrometry (MS) have greatly evolved in the past years, and nowadays it is possible to routinely identify thousands of peptides from complex biological samples in a single LC-MS/MS experiment. Despite the advancements in proteomic technologies, the scientific community still faces important challenges in terms of depth and reproducibility of proteomics analyses. Here, we present a multicenter study designed to evaluate long-term performance of LC-MS/MS platforms within the Spanish Proteomics Facilities Network (ProteoRed-ISCIII). The study was performed under well-established standard operating procedures, and demonstrated that it is possible to attain high qualitative and quantitative reproducibility over time. Our study highlights the importance of deploying platform quality assessment metrics in multi-laboratory studies in early LC-MS/MS system troubleshooting. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-20 |
AnnouncementXML | Submission_2015-07-20_14:42:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Salvador Martinez-Bartolome |
SpeciesList | scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: 1247190; |
ModificationList | Oxidation; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylix acid (Pro); Carbamidomethyl |
Instrument | LTQ Orbitrap Velos; LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2013-04-08 03:41:30 | ID requested | |
⏵ 1 | 2015-07-20 14:42:02 | announced |
Publication List
Campos A, D, í, az R, Mart, í, nez-Bartolom, é S, Sierra J, Gallardo O, Sabid, ó E, L, ó, pez-Lucendo M, Ignacio Casal J, Pasquarello C, Scherl A, Chiva C, Borras E, Odena A, Elortza F, Azkargorta M, Ibarrola N, Canals F, Albar JP, Oliveira E, Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers. J Proteomics, 127(Pt B):264-74(2015) [pubmed] |
Keyword List
submitter keyword: Multicentric, QC, Reproducibility, Orbitrap |
Contact List
Eliandre de Oliveira | |
---|---|
contact affiliation | ProteoRed-ISCIII, Proteomics Platform, Barcelona Science Park, Barcelona, Spain. |
contact email | eoliveira@pcb.ub.es |
lab head | |
Salvador Martinez-Bartolome | |
contact affiliation | ProteoRed-ISCIII, Proteomics Facility, Centro Nacional de Biotecnología - CSIC, Madrid, Spain. |
contact email | smartinez@proteored.org |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD000205 |
PRIDE project URI |
Repository Record List
[ + ]