Updated project metadata. Splenocytes from 14-month Alzheimer's Disease mouse model were separated with CD90.2 magetic beads into two cell subsets: CD90+ and CD90-. Proteome for these two subset were characterized with offline SCX-nanoLC-MS/MS. MS data was analyzed with Proteome Discoverer 1.3 software. MS/MS spectra were searched against the IPI mouse database (August 16, 2012, 59 534 sequences). SEQUEST search parameters were as follows: two maximum trypsin miscleavages; precursor and fragment mass tolerances of 10 ppm and 0.8 Da, respectively; carbamidomethyl modification on cysteine was a static modification; oxidation of methionine was a dynamic modification. Decoy database searching was employed to control the false discovery rate (FDR) and generate lists of peptides with high (FDR < 0.01) and medium (FDR < 0.05) confidence.