PXD000203

PXD000203 is an original dataset announced via ProteomeXchange.

Title	Mouse splenocyte proteome
Description	Splenocytes from 14-month Alzheimer's Disease mouse model were separated with CD90.2 magetic beads into two cell subsets: CD90+ and CD90-. Proteome for these two subset were characterized with offline SCX-nanoLC-MS/MS. MS data was analyzed with Proteome Discoverer 1.3 software. MS/MS spectra were searched against the IPI mouse database (August 16, 2012, 59 534 sequences). SEQUEST search parameters were as follows: two maximum trypsin miscleavages; precursor and fragment mass tolerances of 10 ppm and 0.8 Da, respectively; carbamidomethyl modification on cysteine was a static modification; oxidation of methionine was a dynamic modification. Decoy database searching was employed to control the false discovery rate (FDR) and generate lists of peptides with high (FDR < 0.01) and medium (FDR < 0.05) confidence.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:03:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyun Cao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-04-07 15:01:57	ID requested
1	2014-01-31 01:45:09	announced
⏵ 2	2014-07-25 01:03:02	announced	Updated project metadata.

Cao Z, Robinson RA, pp/ps-1 Alzheimer's disease model. Proteomics, 14(2-3):291-7(2014) [pubmed]

submitter keyword: Alzheimer’s disease, T cells, CD90, splenocytes, APP/PS-1, mice, proteomics

Zhiyun Cao
contact affiliation	University of Pittsburgh
contact email	zhc23@pitt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/01/PXD000203

PRIDE project URI

• PRIDE
  1. PXD000203
     1. Label: PRIDE project
     2. Name: Mouse splenocyte proteome