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PXD000197

PXD000197 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAKT2 interacting proteins_ MBP- and MAP-TAPs
DescriptionTagged AKT2 was expressed in HEK293T cells. For quantification SILAC labeling was performed. MBP-TAP: Cell lysates were mixed at a 1:1 ratio with unlabeled wild type cells before Tandem Affinity Purification. MAP-TAP: Same amount of labeled and unlabeled cell lysates were purified via Tandem Affinity Purification and afterwards eluates were mixed at a 1:1 ratio. AKT2 and co-purified proteins were digested with trypsin, fractionated via SCX and analyzed via LC-MS. Sequence database-search of the MS data was performed against the uniprot human taxonomy-9606 database containing 83659 entries using the SEQUEST algorithm with the following parameters: trypsin specificity, two missed cleavage sites, precurser ion mass accuracy tolerance of 10–30 ppm, cysteine carbamidomethylation, methionine oxidation, pSTY, N-terminal protein acetylation and, when performed, SILAC labels Lys-6, Arg-6 specified as modifications. The minimal cross-correlation score (XCorr) was set to 2.0, 2.5 and 3.0 for charge states +2, +3 and +4 respectively. The Delta Cn had to be >0.1 and the minimal peptide probability allowed was 0.05. The minimum number of peptides necessary for protein identification was three.
HostingRepositoryPRIDE
AnnounceDate2013-08-13
AnnouncementXMLSubmission_2013-08-13_06:20:27.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000197
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKatharina Bottermann
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListO-phospho-L-threonine: 79.966331; O-phospho-L-serine: 79.966331; 6x(13)C labeled L-lysine: 6.020129; iodoacetamide -site C: 57.021464; 6x(13)C labeled L-arginine: 6.020129; oxidation: 15.994915; O4'-phospho-L-tyrosine: 79.966331
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-28 07:04:37ID requested
12013-08-13 04:52:24announced
12014-07-28 00:33:05announced
22013-08-13 06:20:27announcedAdd reference
Publication List
Bottermann K, Reinartz M, Barsoum M, K, ö, tter S, G, ö, decke A, -Enolase as Novel Interaction Partners of AKT2. PLoS One, 8(6):e66045(2013) [pubmed]
Keyword List
submitter keyword: Human, HEK293T, AKT2, TAP, LTQ-Orbitrap
Contact List
Katharina Bottermann
contact affiliationUniversitaet Duesseldorf
contact emailKatharina.Bottermann@uni-duesseldorf.de
Full Dataset Link List
Dataset FTP location
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Repository Record List
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