PXD000189

PXD000189 is an original dataset announced via ProteomeXchange.

Title	Searching for Pseudomonas aeruginosa MagD partners
Description	Opportunistic pathogen Pseudomonas aeruginosa synthesizes a structural homologue of the human alpha2-Macroglobulin protein, a large spectrum protease inhibitor and important player of innate immunity. Delta-MagD and MagD-WT Pseudomonas aeruginosa strains were lysed and lysates submitted to co-immunoprecipitation using anti-MagD antibody (2 biological replicates). Immunoprecipitated proteins were in-gel digested and resulting peptides analysed by nanoLC-MS/MS. Identifications were realised using Mascot and filtered using IRMa software (1% FDR). Results were exported to a relational database (MSIdb) and processed using hEIDI software to identify proteins enriched in MagD-WT samples compared to delta-MagD samples.
HostingRepository	PRIDE
AnnounceDate	2013-08-09
AnnouncementXML	Submission_2013-08-09_07:40:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000189
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228); NCBI TaxID: 208964;
ModificationList	No applicable mass modifications
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-03-22 02:29:14	ID requested
1	2013-08-09 07:31:34	announced
1	2014-07-28 00:31:58	announced
⏵ 2	2013-08-09 07:40:06	announced	Add reference

Robert-Genthon M, Casabona MG, Neves D, Cout, é Y, Cic, é, ron F, Elsen S, Dessen A, Attr, é, e I, 2-macroglobulin homolog. mBio, 4(4):(2013) [pubmed]

submitter keyword: Pseudomonas aeruginosa, co-immunoprecipitation, nanoLC-MS/MS

Yohann Couté
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	yohann.coute@cea.fr

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• PRIDE
  1. 28856
     1. InstrumentRef: INSTRUMENT_1
     2. Label: MagD co-IP
     3. ModificationList: No applicable mass modifications,
     4. Name: Ctrl and test samples
     5. PublicationRef: PMID23919994