<<< Full experiment listing

PXD000183

PXD000183 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman kidney primary proximal tubule cell LC-MSMS
DescriptionAngiotensin II (AngII), the effector of the renin angiotensin system causes kidney disease progression by signalling through AT-1 receptor, but there are no measures of AngII activity in the kidney. Accordingly, we sought to define an AngII-regulated proteome in primary human proximal tubular epithelial cells (PTEC) in order to identify potential markers of AngII activity in the kidney. PTECs were labeled with SILAC heavy arginine(+6) and lysine(+8) OR light arginine and lysine for 6 doubling times. Heavy-labeled PTECs were stimulated with AngII and light-labeled PTECs were stimulated with the control medium. AngII-treated and control-treated PTEC lysates were mixed in 1:1 total protein ratio and their proteomes were compared. We generated 5 biological replicates (1 supernatant, 1 replicate with reverse labeling). LTQ-Orbitrap mass spectrometer was used for LC-MSMS analysis. Of 4618 quantified proteins, 83 were differentially regulated by AngII in 4 biological replicates of PTEC lysates. We subsequently confirmed and verified 18 differentially regulated proteins by using SRM, RT-PCR and ELISA. We also went on to validate the main functional, enriched networks by using systems biology approach and an in vivo mouse model.
HostingRepositoryPRIDE
AnnounceDate2013-07-18
AnnouncementXMLSubmission_2013-07-18_05:45:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAna Konvalinka
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue; 6x(13)C: 2x(15)N labeled L-lysine; 6x(13)C labeled L-arginine; N-acetylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-17 13:28:06ID requested
12013-07-18 05:42:56announced
12014-07-25 00:59:42announced
22013-07-18 05:45:19announcedAdd reference
Publication List
Konvalinka A, Zhou J, Dimitromanolakis A, Drabovich AP, Fang F, Gurley S, Coffman T, John R, Zhang SL, Diamandis EP, Scholey JW, Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC). J Biol Chem, 288(34):24834-47(2013) [pubmed]
Keyword List
submitter keyword: human, kidney, SILAC, proximal tubule epithelial cell, LC-MSMS, angiotensin II
Contact List
Ana Konvalinka
contact affiliationDepartment of the Institute of Medical Sciences, Division of Nephrology
contact emailana.konvalinka@mail.utoronto.ca
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/07/PXD000183