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PXD000182

PXD000182 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis nanodomain-delimited ABA signaling pathway regulates the anion channel SLAH3
DescriptionTwo datasets: StREM1.3: methyl-beta cyclodextrin-dependent distribution of StREM1.3 to detergent resistant and detergent soluble membrane fractions. SLAH3: localization of SLAH3 to detergent resistant and detergent soluble fractions dependent on co-expression with CPK21 and ABI1. Data analysis: Protein identification and ion intensity quantitation was carried out by MaxQuant version 1.3.0.5. Spectra were matched against the Arabidopsis proteome (TAIR10, 35,386 entries) using Andromeda. Thereby, carbamidomethylation of cysteine was set as a fixed modification; oxidation of methionine as well as phosphorylation of serine, threonine, and tyrosine was set as variable modifications. Because label-free quantitation based on ion intensities was used, multiplicity was set to 1. Mass tolerance for the database search was set to 20 ppm on full scans and 0.5 Da for fragment ions. Retention time matching between runs was chosen within a time window of 2 min. Peptide false discovery rate (FDR), protein FDR was set to 0.01, and site FDR was set to 0.05. Contaminants and reverse hits identified by MaxQuant were excluded from further analys Protein identification and ion intensity quantitation was carried out by MaxQuant version 1.3.0.5. Spectra were matched against the Arabidopsis proteome (TAIR10, 35,386 entries) using Andromeda. Thereby, carbamidomethylation of cysteine was set as a fixed modification; oxidation of methionine as well as phosphorylation of serine, threonine, and tyrosine was set as variable modifications. Because label-free quantitation based on ion intensities was used, multiplicity was set to 1. Mass tolerance for the database search was set to 20 ppm on full scans and 0.5 Da for fragment ions. Retention time matching between runs was chosen within a time window of 2 min. Peptide false discovery rate (FDR), protein FDR was set to 0.01, and site FDR was set to 0.05. Contaminants and reverse hits identified by MaxQuant were excluded from further analysis. PXD000211 is also assoicated with the same study/paper.
HostingRepositoryPRIDE
AnnounceDate2013-09-02
AnnouncementXMLSubmission_2013-09-02_02:39:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWaltraud Schulze
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListacetylated residue; iodoacetamide derivatized residue; phosphorylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-15 07:48:09ID requested
12013-09-02 02:31:25announced
12014-07-25 00:59:16announced
22013-09-02 02:39:23announcedAdd reference
Publication List
Demir F, Horntrich C, Blachutzik JO, Scherzer S, Reinders Y, Kierszniowska S, Schulze WX, Harms GS, Hedrich R, Geiger D, Kreuzer I, Arabidopsis nanodomain-delimited ABA signaling pathway regulates the anion channel SLAH3. Proc Natl Acad Sci U S A, 110(20):8296-301(2013) [pubmed]
Keyword List
submitter keyword: membrane microdomain, ion channel, remorin
Contact List
Waltraud Schulze
contact affiliationDepartment of Plant Systems Biology
contact emailwschulze@uni-hohenheim.de
Full Dataset Link List
Dataset FTP location
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